Microbial Inactivation Methods

Dynamical regimes of excitable cells under pulsed electric fields: From excitation to electroporation

This study models the interaction between excitation and electroporation in neurons under nsPEFs, identifying safe regimes for excitation without electroporation and vice versa.

Paper-Based Microfluidic Continuous-Flow Electroporation (μCEP) for Bacterial Transformation through Pore-Scale Electric-Field Enhancement

This paper introduces a low-cost, scalable microfluidic electroporation technique for bacterial transformation with improved cell viability and efficiency compared to traditional cuvette-based methods.

Resolving pulsed electric field effects on proteins in bulk aqueous solution

This study investigates how pulsed electric fields affect proteins in bulk solution, showing that pH changes, not direct field-protein interactions, cause persistent structural and functional changes in tubulin.

Increased Ovarian and Colorectal Cancer Cell Sensitivity to Platinum Drugs and innovative TS Inhibitors by Electroporation

This study evaluates the effect of electrochemotherapy (ECT) on ovarian and colorectal cancer cell lines using platinum drugs and thymidylate synthase inhibitors, finding that ECT enhances drug efficacy by increasing cellular uptake.

Electroporation-mediated delivery of protein biosensors for metabolic imaging in differentiated myotubes

The paper develops an electroporation method to deliver protein biosensors into differentiated myotubes for real-time metabolic imaging.

Endoluminal catheter pulsed field ablation for the treatment of atherosclerotic vascular disease

This paper demonstrates that pulsed field ablation can selectively ablate atherosclerotic plaques while preserving extracellular structure in ex vivo models.

Simple Electroporation of Chlamydomonas reinhardtii Strains with an Intact Cell Wall

The paper describes a simple, fast, and efficient electroporation protocol for Chlamydomonas reinhardtii strains with an intact cell wall, achieving high transformation efficiency without cell wall digestion or commercial kits.

Microparticle-Enabled Single Cell Multiparameter Electronic Immunophenotyping for Selective Electroporation

The paper introduces ME-SPICy, a microfluidic platform for targeted electroporation of selected cell subpopulations using antibody-conjugated microparticles and impedance detection, achieving 5-fold enrichment of lymphocytes.

Novel combination of irreversible electroporation and allogenic chimeric antigen receptor (CAR) T-cell therapy synergizes therapeutic outcomes in a preclinical human pancreatic cancer mouse model

This study combines irreversible electroporation with CAR T-cell therapy to improve outcomes in a pancreatic cancer mouse model by targeting residual tumor cells post-ablation.

Membrane Geometric Confinement Reshapes the Lateral Electric Field Distribution and Intracellular Cargo Transport in Nanopore Electroporation

This study shows that membrane geometry in nanopore electroporation affects electric field distribution and cargo transport, enabling controlled delivery patterns.

Label-Free Targeted High Efficiency Electroporation with Single-Cell Feedback Control Using Focused Microscale Electric Fields

This paper introduces SPICy, a label-free, continuous-flow electroporation system that enables high-efficiency, cell-selective cargo delivery with high viability and low voltage.

Giant pore formation in vesicles under msPEF-induced electroporation: role of charging time and waveform

This study investigates how charging time affects giant pore formation in vesicles under msPEF-induced electroporation, showing delays in pore formation and increased giant pore area with longer charging times.

Low-pressure Isochoric Freezing as a scalable technique for fresh food preservation

This study demonstrates that low-pressure isochoric freezing can inhibit microbial growth and preserve food quality for up to 5 weeks using raw cow's milk as a model.

A microfluidic platform for in situ studies of bacteria electroporation

This paper introduces a microfluidic platform for single-cell electroporation with in situ microscopy and demonstrates DNA delivery into bacteria, highlighting key factors affecting electroporation efficiency.

Optimizing Electroporation Parameters for Efficient Delivery of Large Molecules into Pig Zygotes Using Fluorescent Dextrans from 3 to 2000 kDA

This study optimizes electroporation parameters for delivering large fluorescent dextrans into pig zygotes, finding voltage and polarity effects on molecule permeability.