Legume Nitrogen Fixing Symbiosis

Isolation of rhizobia from Ontario soils that are effective at fixing nitrogen with common bean (Phaseolus vulgaris)

The paper isolates and characterizes rhizobia from Ontario soils that form symbiotic relationships with common bean, identifying diverse species and high-performing strains for potential use in inoculants.

Kinetic model of a determinate legume root nodule reveals plant metabolic characteristics for more efficient nitrogen fixation symbiosis

The paper develops a kinetic model of legume root nodule metabolism to identify metabolic targets for improving nitrogen fixation efficiency and rate.

The free-living wellspring of symbiotic nitrogen fixation in Bradyrhizobium

This paper identifies free-living Bradyrhizobium as the ancestral state of nitrogen fixation, showing that symbiotic nitrogen fixation evolved multiple times independently from free-living ancestors.

Characterization of chemotaxis in soybean symbiont Bradyrhizobium diazoefficiens

This study identifies cheA2 as the primary chemotaxis driver in Bradyrhizobium diazoefficiens toward soybean seed exudate, revealing genomic context effects and chemotaxis independence from swimming speed.

Spatiotemporal dynamics of ethylene biosynthesis shape infection and nodule initiation in Medicago truncatula

This study shows how ethylene biosynthesis shifts spatially during nodule initiation in Medicago truncatula, with specific ACS genes regulating infection and nodule formation.

Rhizobia independently adapt to soil and legume host environments, but soil conditions influence the abundance of high-quality partners.

This study shows rhizobia adapt independently to soil and legume host environments, but soil conditions affect the abundance of high-quality nitrogen-fixing strains.

COCHLEATA controls spatial regulation of cytokinin and auxin during nodule development

This study shows COCHLEATA regulates auxin and cytokinin levels during nodule development, with PsCOCH mutants showing altered hormone responses and gene expression profiles.

Pisum sativum applies sanctions to less effective strains at the nodule level, but not within mixed nodules

This study shows that pea plants sanction entire nodules based on nitrogen fixation rates, not individual bacteria, preventing ineffective strains from evading sanctions by piggybacking.

Gene family expansions and nodule-specific expression patterns reveal the recruitment of Beta-Glucosidases and Cytochrome P450 genes to nodulation in soybean

Cyclophilin A-mediated cis/trans isomerization modulates RIN4 to control intracellular rhizobial infection in legumes

This study shows Cyclophilin A modulates RIN4 conformation to control legume infection by rhizobia, revealing a host-symbiont interaction mechanism.

RHD6LA regulates root hair responses to both symbionts and commensals

The paper identifies RHD6LA as a gene required for root hair responses to both symbiotic rhizobia and commensal soil bacteria using single-cell RNA sequencing.

Engineering and evaluation of Sinorhizobium meliloti nodulation (nod) gene reporter systems in rhizobia and non-rhizobia

The paper develops inducible nodulation gene reporters to identify rhizobial and non-rhizobial strains capable of expressing S. meliloti nod genes, aiding in synthetic nitrogen fixation in non-legumes.

Distinct Proline residues in hinge-regions of SYMRK generates a phosphocode for releasing Malectin-like-Domain to allow progress of rhizobia-legume symbiosis at epidermal-cortical barrier.

The paper identifies two distinct Proline residues in SYMRK hinge regions that enable MLD release, facilitating rhizobia-legume symbiosis by overcoming the epidermal-cortical barrier.

A single small RNA shapes multiple symbiotic traits in rhizobia

This study identifies NfeR1, a small RNA that regulates multiple symbiotic traits in Rhizobium by targeting mRNAs involved in nitrogen metabolism, cell cycle, and nodulation.

Simulated drought with Polyethylene-Glycol (PEG) decreases above-ground performance and increases nodulation in the legume Medicago lupulina

This study shows that PEG-simulated drought reduces above-ground growth in Medicago lupulina but increases nodulation, suggesting PEG may not accurately simulate drought effects on rhizobial symbiosis.

Soybean Root Nodule Occupancy: Competition Between Bradyrhizobium and Sinorhizobium Strains Inoculated at Different Plant Growth Stages

This study investigates how the age of soybean plants at inoculation affects the selection of Bradyrhizobium or Sinorhizobium strains in root nodules.

Exploring symbiotic legume-rhizobia relationships across tropical species

The paper examines legume-rhizobia symbiosis across 109 tropical species, identifying predictors of nodulation and associated bacterial genera.

Rhizobial motility preference in root colonization of Medicago truncatula

This study investigates how rhizobia move within root infection threads using modeling, imaging, and mutant analysis, finding that flagella-independent surface translocation and rhizobactin 1021 secretion are key for colonization.

Rhizobial effector NopM ubiquitinates Nod factor receptor NFR5 and promotes rhizobial infection in Lotus japonicus

This study identifies NopM as a rhizobial effector that ubiquitinates NFR5, enhancing infection of Lotus japonicus by modulating Nod factor signaling.

Concurrent ecological and evolutionary processes contribute to mutualism breakdown between legumes and rhizobia

The paper examines how ecological and evolutionary processes interact to cause mutualism breakdown between legumes and rhizobia over 33 years of nitrogen fertilization.

Transitive interactions among rhizobia determine their symbiotic fitness

The paper quantifies how transitive bacterial interactions affect rhizobial symbiotic fitness during legume infection.

Mutations in legume genes that influence symbiosis create a complex selective landscape for rhizobial symbionts

This study investigates how host mutations affect rhizobial fitness by inoculating Medicago truncatula mutants with Sinorhizobium meliloti strains, revealing host-by-strain interactions and identifying key rhizobial genes involved in fitness responses.

Diversity of exopolysaccharide cluster I in the Bradyrhizobium genus

The paper examines the presence of EPS cluster I in Bradyrhizobium strains, finding it absent in B. elkanii and Photosynthetic Bradyrhizobium supergroups.

Disruption of putrescine export in experimentally evolved Ralstonia pseudosolanacearum enhances symbiosis with Mimosa pudica

This study shows that disrupting putrescine export in Ralstonia pseudosolanacearum enhances its symbiosis with Mimosa pudica by altering host gene expression and increasing bacterial load in nodules.

The Lotus japonicus alpha Expansin EXPA1 is recruited during intracellular and intercellular rhizobial colonization

This study shows that LjEXPA1 is essential for both intracellular and intercellular rhizobial colonization in Lotus japonicus by remodeling cell walls during symbiosis.

Plasmid transmission dynamics and evolution of partner quality in a natural population of Rhizobium leguminosarum

The paper analyzes plasmid transmission dynamics in Rhizobium leguminosarum, finding variation in plasmid types and their association with partner quality decline due to nitrogen fertilization.

Two residues reprogram immunity receptor kinases to signal in nitrogen-fixing symbiosis

The paper identifies two amino acid residues in NFR1 that determine signalling specificity between immune and symbiotic responses in legume root cells.

Phloem-Specific Translational Regulation of Soybean Nodulation: Insights from a Phloem-Targeted TRAP-Seq Approach

This study uses TRAP-seq to analyze phloem-specific translational dynamics during soybean nodulation, identifying key regulators and pathways involved in the process.

Rhizobial infection-specific accumulation ofphosphatidylinositol 4,5-bisphosphate inhibits the excessive infection of rhizobia in Lotus japonicus

The paper identifies that PI(4,5)P2 accumulation suppresses excessive rhizobial infection in Lotus japonicus roots, leading to optimal nodule formation.

Identification of regulatory promoter sequences directing MtCP6 transcription at the onset of nodule senescence in Medicago truncatula.

The paper identifies a cis-regulatory sequence, NS-box, that directs MtCP6 transcription during nodule senescence in Medicago truncatula and shows its interaction with an ERF transcription factor.