Anna N. Stepanova

An Integrated Spatially Resolved Mechanistic Model of Hierarchical Auxin-Cytokinin-Ethylene Crosstalk Underlying Root Growth Inhibition in Arabidopsis

The paper develops ACE and ACE2 reporters to study hormone interactions in Arabidopsis roots, revealing spatial and temporal auxin-cytokinin-ethylene crosstalk mechanisms.

Spatiotemporal dynamics of ethylene biosynthesis shape infection and nodule initiation in Medicago truncatula

This study shows how ethylene biosynthesis shifts spatially during nodule initiation in Medicago truncatula, with specific ACS genes regulating infection and nodule formation.

Arabidopsis lines with modified ascorbate concentrations reveal a link between ascorbate and auxin biosynthesis

The paper investigates the relationship between ascorbate levels and auxin biosynthesis in Arabidopsis by analyzing mutant lines with altered ascorbate concentrations.

The CYP71A, NIT, AMI, and IAMH gene families are dispensable for indole-3-acetaldoxime-mediated auxin biosynthesis in Arabidopsis

This study shows that gene families involved in the IAOx pathway are not essential for auxin biosynthesis in Arabidopsis, challenging the proposed linear pathway and suggesting an uncharacterized route.

EBSn, a robust synthetic reporter for monitoring ethylene responses in plants

The paper develops and tests EBSn, a synthetic ethylene-inducible promoter that outperforms existing reporters in sensitivity and tissue coverage in Arabidopsis and tomato.

Development of modular geminivirus-based vectors for high cargo expression and gene targeting in plants

The paper develops a modular geminivirus-based vector system for improved protein expression and gene targeting in plants, showing that native promoters and removal of virion-sense genes enhance reporter expression without affecting replicon accumulation.

DASH: A versatile and high-capacity gene stacking system for plant synthetic biology

The paper introduces DASH, a gene stacking system for plant synthetic biology that overcomes limitations of existing methods by combining Golden Gate, recombineering, and recombinase systems.

Targeted DNA ADP-ribosylation triggers templated repair in bacteria and base mutagenesis in eukaryotes

This paper introduces append editing, a method that uses ADP-ribosylation to achieve distinct genome editing outcomes in bacteria and eukaryotes.

Structure-function analysis of interallelic complementation in ROOTY transheterozygotes

The paper identifies hypomorphic mutants of the ROOTY gene in plants and shows interallelic complementation in transheterozygotes, supported by structural modeling of RTY protein interactions.