Reproductive biology and impacts on aquatic species

This study identifies paternal-effect genes in fish by comparing progeny from fresh and cryopreserved semen, finding that cryopreservation upregulates genes involved in visual development.

The paper develops methods to cryopreserve sperm from two sea urchin species, maintaining fertilization capacity and producing viable larvae after multiple freeze-thaw cycles.

The paper develops a Real-Time PCR protocol for sex identification in Atlantic salmon, demonstrating accuracy and applicability to degraded DNA samples.

This study investigates the effects of dre-miR-210-5p in zebrafish ovarian cells using an in vitro model, identifying thousands of differentially expressed genes and their involvement in reproductive and immune pathways.

The paper identifies bouncer as a key protein enabling immature oocyte fertilization in guppies, a unique reproductive strategy not observed in other fish species.

This study uses CRISPR/Cas9 to disrupt vtg2 in zebrafish, revealing its essential role in vitellin membrane formation and early embryonic survival.

This study shows that in medaka, photoperiod regulates gonadotrope cell division and hormone production through melatonin, TSH, and folliculostellate cells.

This study characterizes ovarian development stages and identifies VgR gene silencing as a factor reducing fecundity in parthenogenetic Artemia.

This paper demonstrates the sterilization of Atlantic salmon embryos using antisense oligonucleotides targeting the dead end gene, resulting in germ cell-depleted fish with normal morphology but no gametes.

This study shows that bmp15 deficiency in zebrafish causes follicle arrest and sex reversal, which can be rescued by inhibin mutation, revealing interactions between bmp15 and the activin-inhibin system in folliculogenesis.

The paper develops antibodies and an immunofluorescence method to accurately identify spermatogenic cell types in fish testis samples across multiple species.

This study identifies differences in proteomic profiles between good and poor quality Atlantic halibut eggs, showing impaired transcription and protein folding in poor quality eggs affecting early development.

The paper identifies sex-specific genes in sea urchin gonads that are expressed before metamorphosis, revealing early sex determination and unique gonad-specific transcripts.

The paper identifies genes in the germline-specific region of the sea lamprey genome and shows their enrichment in testis expression and potential role in sex determination.

This study identifies ndrg1b as a regulator of cystic proliferation in medaka gonads, showing its role in gamete production and reproductive behavior.

The paper identifies regulators of sexual maturation in Atlantic salmon by integrating transcriptome, methylome, and chromatin accessibility data from a time course experiment.

This study shows that zebrafish dazl is essential for cystogenesis, germline stem cell specification, and fertility by regulating incomplete cytokinesis and meiotic entry.

The paper analyzes the gonad transcriptome of golden mussel Limnoperna fortunei to identify sex differentiation genes, finding 15 male-biased and 4 female-biased homologs of known sex-related gene families.

This paper presents the first gonadal transcriptome analysis of Gobiopterus lacustris, identifying differentially expressed genes and pathways involved in gonadal development and reproduction.

This study investigates the role of Amh in zebrafish gonad development and sex determination by generating amh null mutants, revealing its involvement in germ cell regulation and gonadal soma development.

This study shows that retinoic acid promotes spermatogonial differentiation and reduces spermatid apoptosis in zebrafish, but is not required for meiosis, with androgens partially compensating for RA loss.

The paper analyzes the transcriptome of Culter alburnus gonad tissues to identify sex-related genes and pathways using Illumina sequencing.

The paper identifies gene signatures in zebrafish eggs that predict developmental success and discovers two new maternal-effect genes involved in vertebrate reproduction.

This study shows that melengestrol acetate exposure impairs zebrafish reproduction by reducing egg production and hormone levels.