Tech Note- Increased Complexity of Amplicon Libraries using Phased Primers
The paper describes how phased primers increase amplicon library complexity, improving sequencing quality and reducing PhiX usage.
363,000+ bioRxiv and medRxiv preprints, including those later published in peer-reviewed journals, across 26 biological disciplines.
The paper describes how phased primers increase amplicon library complexity, improving sequencing quality and reducing PhiX usage.
Nikunj Goel, Christen M. Bossu, Justin J. Van Ee…·Mevin B. Hooten
The paper develops a hierarchical Bayesian model to identify local adaptation by accounting for observational uncertainty and evolutionary processes, finding 30 genes associated with temperature seasonality in Willow Flycatchers.
Adam P. Ryan, Sean A. Bergin, Jillian Scully…·Geraldine Butler
The paper defines the pangenome of 372 Candida parapsilosis isolates, revealing low genetic diversity and identifying gene content variations associated with nitrogen metabolism.
Virginia Baraja-Fonseca, Andrea Arrones, Santiago Vilanova…·Pietro Gramazio
This study benchmarks low-coverage sequencing workflows for eggplant genotyping, finding that 3X coverage with Freebayes achieves high genotypic concordance and sensitivity.
Rachael Cowan, Alyssa Koehler, Clairine I. S. Larsen…·Lori Boies
The paper characterizes the Ilp4 ortholog in Drosophila grimshawi as part of a dataset studying IIS pathway evolution.
Heng Li, Tianchun Xue, Rachel Patton McCord
This study measures radiation-induced DNA damage distribution using END-seq and shows its correlation with 3D genome structure and chromatin features.
Yutang Chen, Jenny Kiesbauer, Dario Copetti…·Bruno Studer
The paper presents a chromosome-level haplotype-resolved genome assembly for Italian ryegrass, revealing gene order collinearity and sequence variation between haplotypes, and identifies QTL for stem rust resistance.
Leslie A. Guerrero, Kenzie N. Pollard, Rachael A. Bay
This study examines chromatin accessibility in the coral Acropora millepora under heat stress, finding that open chromatin regions correlate with gene expression patterns and stress responses.
Philip C. Dishuck, Katherine M. Munson, Alexandra P. Lewis…·Evan E. Eichler
The paper characterizes the structural variation and diversification of the NPIP gene family in humans using long-read sequencing and identifies evidence of positive selection and novel gene models.
Ben J. G. Sutherland, Konstantin Divilov, Neil F. Thompson…·Timothy J. Green
The paper demonstrates genome-wide imputation using a low-density amplicon panel in Pacific oysters to reduce genotyping costs and improve selective breeding.
Jolanthe Lingeman, Diana van den Heuvel, Jeroen van den Berg…·Haico van Attikum
This study shows the NSL complex prevents R-loop induced replication stress during neural development, leading to developmental abnormalities when disrupted.
Istiaque Zaeem, Nurnabi Azad Jewel, Mohimenul Haque Rolin…·Shakhinur Islam Mondal
This study identifies wCin2USA1 as a promising Wolbachia strain for dengue control due to its genomic similarities to established strains and unique Cif gene architecture.
Nolan G. Gokey, James M. Ward, Eric J. Milliman…·Trevor Archer
The paper shows that loss of H1.4 linker histone affects chromatin accessibility, histone modifications, and gene expression, particularly impacting stress response pathways and immune-related genes.
Luz García‐Longoria, Vaidas Palinauskas, Justé Aželytė…·Olof Hellgren
This study investigates how the avian malaria parasite P. homocircumflexum adjusts its gene expression across different bird hosts, finding evidence for epigenetic inheritance and transcriptional plasticity.
Javier Gancedo-Verdejo, Rocío G. Urdinguio, Juan Ramón Tejedor…·Mario F. Fraga
The paper shows that environmental enrichment modulates age-related changes in the 3D chromatin interactome of mouse hippocampal neurons, inducing maturation in young mice and partial rejuvenation in old mice.
Jiale Chen, Xianli Gao, Chunhua Zhang, Ying Ge
The paper expands the BacMet database and trains an enhanced ResNet model to rapidly identify metal resistance genes with high accuracy and speed.
Antonio Carvajal‐Rodríguez, Sara Rocha, Marina Pampín…·Armando Caballero
KLinterSel evaluates concordance among selective sweep detection methods using statistical tests and identifies candidate site clusters in common cockle genomic data.
Dailu Guan, Jennifer Jéssica Bruscadin, Wen Yang…·Huaijun Zhou
The paper constructs a bovine epigenomic atlas to annotate regulatory elements and decode the regulatory syntax of the cattle genome, aiding in fine-mapping GWAS loci for complex traits.