Discover, rate, and discuss biology preprints.

The paper shows human neutrophils release extracellular RNAs that modulate gene expression in Aspergillus fumigatus, independent of fungal argonaute proteins.

The paper benchmarks microbiome methods to improve metagenomic sequencing of lower respiratory tract samples by optimizing DNA purification strategies.

This study identifies agaSY genes in UPEC as essential for amino sugar catabolism and links their loss to reduced virulence and colonization in a murine model.

This study introduces fungal-DBiT, a spatial transcriptomic platform that maps host and fungal RNAs in tissue sections, revealing spatial heterogeneity in fungal localization and host responses during infection and under physiological conditions.

This study shows that HCMV miRNAs miR-US5-2 and miR-US25-2 downregulate Dvl3 during latency to enable efficient reactivation in CD34+ HPCs.

This study identifies c-type cytochrome biogenesis as a conserved target of copper toxicity in bacteria, showing Cu disrupts assembly of these complexes across diverse species.

The paper studies RNA phage infection dynamics in E. coli, showing bacterial population rebound despite viral replication and cell death.

This study examines phylosymbiosis in bees, finding moderate patterns with lineage-specific microbial communities and weak ecological trait effects on microbiomes.

The paper investigates how alternative respiratory electron transport pathways affect aminoglycoside susceptibility in bacteria.

This study evaluates how alternating polarity frequency affects the assembly of electro-methanogenic microbial communities, finding that 4-day alternation yields the best performance.

The paper identifies a novel mycovirus in clinical isolates of Cryptococcus neoformans, showing its presence in six isolates from HIV-positive patients with meningitis.

The paper creates a synthetic MALDI-TOF MS atlas to improve microbial classification across hospitals by addressing dataset fragmentation and variability.

The paper optimizes a flow cytometry sorting-sequencing workflow to reduce contamination and bias in microbial community analysis.

This study shows the Kappa prophage provides a competitive fitness advantage to Vibrio cholerae through phage predation, but its spread is restricted by BREX and AbiC phage defense systems.

The paper identifies the role of the PilC/PilY1 plug region in T4a pilus biogenesis, showing it interacts with PilK to stabilize the protein and enable pilus formation after filament assembly.

This study shows Dehalococcoides can dechlorinate HCBD through multiple intermediates, identifies a candidate gene, and supports bioremediation strategies for HCBD-contaminated sites.

The paper investigates pressure effects on microbial activity in the Puerto Rico Trench, finding that piezophily dominates subseafloor communities below 20 meters below seafloor.

This study identifies substrates that C. thermocellum can transport and metabolize, revealing insights into its redox balance and fermentation product formation for biofuel production.

This study shows that different antibiotics create distinct plasmid transfer dynamics, revealing drug-specific residual conjugation windows that are decoupled from growth inhibition.