A. Remeeva

Reengineering of a flavin-binding fluorescent protein using ProteinMPNN

The paper tests ProteinMPNN's ability to reengineer a flavin-binding fluorescent protein, showing it can generate variants with similar properties to the original.

Two distinct mechanisms of flavoprotein spectral tuning revealed by low-temperature and time-dependent spectroscopy

The paper uses spectroscopy and X-ray structures to reveal two distinct mechanisms by which flavin-binding proteins tune their fluorescence spectra.

luxA gene from Enhygromyxa salina encodes a functional homodimeric luciferase

The study identifies EsLuxA from Enhygromyxa salina as a functional homodimeric luciferase that produces light in vitro and in vivo, suggesting a simplified evolutionary pathway for bioluminescence.

Fine spectral tuning of a flavin-binding fluorescent protein for multicolor imaging

The paper develops twenty-two finely tuned flavin-binding fluorescent proteins for multicolor imaging by modifying the chromophore's spectral properties.

Insights into the mechanisms of LOV domain color tuning from a set of high-resolution X-ray structures

The paper investigates how mutations of Gln148 in LOV domains affect their absorption spectra and structure, revealing blue shifts and pH sensitivity in certain variants.

Structural and mechanistic insight into spectral tuning in flavin-binding fluorescent proteins

The paper identifies structural and mechanistic causes of spectral tuning in flavin-binding fluorescent proteins through mutagenesis, X-ray crystallography, and computational methods, enabling two-color microscopy.

NanoFAST: Structure-based design of a small fluorogen-activating protein with only 98 amino acids

The paper designs a small fluorogen-activating protein (nanoFAST) by truncating FAST based on structural analysis, resulting in the shortest known genetically encoded tag with 98 amino acids.