Author
Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.
10 papers
Recent·Most cited
A. Nikolaev, A. Kuzmin, E. Markeeva, E. Kuznetsova, O. Semenov, A. Anuchina, A. Remeeva, I. Gushchin
The paper tests ProteinMPNN's ability to reengineer a flavin-binding fluorescent protein, showing it can generate variants with similar properties to the original.
bioRxiv · Aug 25, 2023Bioengineering
A. Nikolaev, E. V. Tropina, K. N. Boldyrev, E. G. Maksimov, V. Borshchevskiy, A. Mishin, A. Yudenko, A. Kuzmin, E. Kuznetsova, O. Semenov, A. Remeeva, I. Gushchin
The paper uses spectroscopy and X-ray structures to reveal two distinct mechanisms by which flavin-binding proteins tune their fluorescence spectra.
bioRxiv · Jul 17, 2023Biophysics
A. Yudenko, S. V. Bazhenov, V. A. Aleksenko, I. M. Goncharov, O. Semenov, A. Remeeva, V. V. Nazarenko, E. Kuznetsova, V. V. Fomin, M. N. Konopleva, R. Al Ebrahim, N. N. Sluchanko, Y. Ryzhykau, Y. S. Semenov, A. Kuklin, , I. Gushchin
The study identifies EsLuxA from Enhygromyxa salina as a functional homodimeric luciferase that produces light in vitro and in vivo, suggesting a simplified evolutionary pathway for bioluminescence.
bioRxiv · Feb 27, 2023Biochemistry
A. Nikolaev, A. Yudenko, A. Smolentseva, A. Bogorodskiy, F. Tsybrov, V. Borshchevskiy, S. Bukhalovich, V. V. Nazarenko, E. Kuznetsova, O. Semenov, A. Remeeva, I. Gushchin
The paper develops twenty-two finely tuned flavin-binding fluorescent proteins for multicolor imaging by modifying the chromophore's spectral properties.
bioRxiv · Dec 9, 2022Biophysics
E. Lyapina, E. Marin, A. Gusach, P. Orekhov, A. Gerasimov, A. Luginina, D. Vakhrameev, M. Ergasheva, M. Kovaleva, G. Khusainov, P. Khorn, M. Shevtsov, K. Kovalev, I. Okhrimenko, P. Popov, , V. Cherezov
The paper presents a high-resolution structure of the S1P5 receptor complexed with an inverse agonist, revealing structural features that explain receptor subtype selectivity and inverse agonism.
bioRxiv · Feb 25, 2022Biophysics
A. Kuzmin, P. Orekhov, R. Astashkin, V. Gordeliy, I. Gushchin
This study uses molecular dynamics simulations to analyze the structure and dynamics of the SARS-CoV-2 envelope protein monomer, revealing its membrane orientation and interactions with host factors.
bioRxiv · Mar 10, 2021Biophysics
A. Remeeva, V. V. Nazarenko, K. Kovalev, I. M. Goncharov, A. Yudenko, R. Astashkin, V. Gordeliy, I. Gushchin
The paper investigates how mutations of Gln148 in LOV domains affect their absorption spectra and structure, revealing blue shifts and pH sensitivity in certain variants.
bioRxiv · Feb 5, 2021Biophysics
K. Roellen, J. Granzin, A. Remeeva, M. D. Davari, T. Gensch, V. V. Nazarenko, K. Kovalev, A. Bogorodskiy, V. Borshchevskiy, S. Hemmer, U. Schwaneberg, V. Gordeliy, K.-E. Jaeger, R. Batra-Safferling, I. Gushchin, U. Krauss
The paper identifies structural and mechanistic causes of spectral tuning in flavin-binding fluorescent proteins through mutagenesis, X-ray crystallography, and computational methods, enabling two-color microscopy.
bioRxiv · Jan 8, 2021Biophysics
K. Kovalev, R. Astashkin, I. Gushchin, P. Orekhov, D. Volkov, E. Zinovev, E. Marin, M. Rulev, A. Alekseev, A. Royant, P. Carpentier, S. Vaganova, C. Baeken, I. Sergeev, D. Zabelskii, , V. Gordeliy
The paper elucidates the molecular mechanism of Na+ pumping by KR2 rhodopsin using crystal structures and molecular dynamics, revealing a switching mechanism and structural similarities to channelrhodopsins.
bioRxiv · Jan 29, 2020Biophysics
K. Kovalev, D. Volkov, R. Astashkin, A. Alekseev, I. Gushchin, J. Haro-Moreno, A. Rogachev, T. Balandin, V. Borshchevskiy, A. Popov, G. Bourenkov, E. Bamberg, F. Rodriguez-Valera, G. Bueldt, V. Gordeliy
The paper presents high-resolution structures of two HeR-48C12 states, revealing unique features and suggesting potential enzymatic activity and involvement in redox processes.
bioRxiv · Sep 12, 2019Biophysics