V. Borshchevskiy

CryoRhodopsins: a comprehensive characterization of a group of microbial rhodopsins from cold environments

The paper characterizes CryoRhodopsins from cold environments, revealing their dual functionality and structural adaptations to low temperatures.

Two distinct mechanisms of flavoprotein spectral tuning revealed by low-temperature and time-dependent spectroscopy

The paper uses spectroscopy and X-ray structures to reveal two distinct mechanisms by which flavin-binding proteins tune their fluorescence spectra.

Monitoring GPCR Conformation with GFP-Inspired Dyes

This study develops GFP-inspired dyes to monitor ligand-induced conformational changes in GPCRs, showing fluorescence responses to agonists and allosteric modulators.

Revealing the Oligomerization of Channelrhodopsin-2 in the Cell Membrane using Photo-Activated Localization Microscopy

This study uses qPALM to show ChR2 predominantly forms dimers in the cell membrane, with disulfide bonds not essential for dimerization and partial monomerization observed at low concentrations.

Fine spectral tuning of a flavin-binding fluorescent protein for multicolor imaging

The paper develops twenty-two finely tuned flavin-binding fluorescent proteins for multicolor imaging by modifying the chromophore's spectral properties.

Structural insights into 3Fe-4S ferredoxins diversity in M.tuberculosis highlighted by a first redox complex with P450.

The paper solves the structures of 3Fe-4S ferredoxins from M. tuberculosis and their complex with CYP143, revealing structural and functional insights into redox interactions.

Structural basis for receptor selectivity and inverse agonism in S1P5 receptors

The paper presents a high-resolution structure of the S1P5 receptor complexed with an inverse agonist, revealing structural features that explain receptor subtype selectivity and inverse agonism.

Structural and mechanistic insight into spectral tuning in flavin-binding fluorescent proteins

The paper identifies structural and mechanistic causes of spectral tuning in flavin-binding fluorescent proteins through mutagenesis, X-ray crystallography, and computational methods, enabling two-color microscopy.

NanoFAST: Structure-based design of a small fluorogen-activating protein with only 98 amino acids

The paper designs a small fluorogen-activating protein (nanoFAST) by truncating FAST based on structural analysis, resulting in the shortest known genetically encoded tag with 98 amino acids.

Sub-millisecond conformational dynamics of the A2A adenosine receptor revealed by single-molecule FRET

This study uses smFRET to analyze the conformational dynamics of the A2A adenosine receptor, revealing slow and fast dynamics in different ligand-bound states.

A new twist of rubredoxin function in M. tuberculosis

This study identifies RubB as a cofactor for mycobacterial cytochrome P450s in M. tuberculosis, showing its role in bacterial survival and potential biotech applications.

Accessing mitochondrial protein import in living cells by protein microinjection

This study develops a microinjection method to study mitochondrial protein import in living cells and finds that puromycin enhances import rates by releasing nascent polypeptides from ribosomes.

Hydroxylation of antitubercular drug candidate, SQ109, by mycobacterial cytochrome P450

The paper shows that Mycobacterium tuberculosis CYP124 hydroxylates SQ109, a drug candidate, and potentially affects its interaction with MmpL3.

Metabolic fate of human immunoactive sterols in Mycobacterium tuberculosis

The paper identifies Mtb enzymes that metabolize human immune oxysterols and vitamin D3 derivatives, revealing a potential drug target for tuberculosis treatment.

Molecular mechanism of light-driven sodium pumping

The paper elucidates the molecular mechanism of Na+ pumping by KR2 rhodopsin using crystal structures and molecular dynamics, revealing a switching mechanism and structural similarities to channelrhodopsins.

High Resolution Structural Insights into Heliorhodopsin Family

The paper presents high-resolution structures of two HeR-48C12 states, revealing unique features and suggesting potential enzymatic activity and involvement in redox processes.