Superhelicase-driven isothermal amplification of repetitive and low-complexity DNA
Fahad Rashid, Jimin Kang, Tiantian Shang, Peter J. Murray, Xiaotian Bi, Raquel Merino‐Urteaga, Momcilo Gavrilov, Wonyoung Jo, Arman Ahmed, Qianyun Yan, Tural Aksel, Doug Barrick, James M. Berger, Taekjip Ha
This study demonstrates that SHARP, a helicase-driven isothermal amplification method, effectively amplifies repetitive and compositionally extreme DNA sequences that are challenging for conventional PCR.
bioRxiv · Nov 27, 2024 · PreprintBiophysics