Fahad Rashid

This study shows SF1 helicase Rep translocates processively on ssDNA and, unexpectedly, also on dsDNA, with high speed and processivity, challenging existing models of helicase function.

DNAsight automates AFM image analysis of chromatin organization, revealing protein-specific structural signatures and nucleosome spacing distributions.

This study develops a high-resolution assay to show that the replisome, composed of helicase and DNA polymerase, is a powerful torsional motor that regulates DNA replication stalling and reactivation.

This study investigates the mechanisms of DNA and RNA strand transfer and protein exchange between SSB and RPA using smFRET, revealing dynamic interactions and length-dependent behavior.

The paper develops a single-molecule platform to study DNA supercoiling effects on protein-DNA interactions, finding that negative supercoiling increases Cas9 off-target effects and enhances MutS binding to mismatched bases.

This study demonstrates that SHARP, a helicase-driven isothermal amplification method, effectively amplifies repetitive and compositionally extreme DNA sequences that are challenging for conventional PCR.