Taekjip Ha

The paper shows that FACT facilitates Ubp10 release from nucleosomes after H2B deubiquitination, revealing release as the rate-limiting step for Ubp10 turnover.

This study uses MINFLUX nanoscopy to show nuclear speckles are hierarchically organized into dynamic nanoscale interaction networks that regulate molecular dynamics.

The paper identifies a distributed architectural code involving cytoskeletal, membrane, and lipid regulators that governs nuclear reshaping during DNA repair.

This study shows SF1 helicase Rep translocates processively on ssDNA and, unexpectedly, also on dsDNA, with high speed and processivity, challenging existing models of helicase function.

This paper develops a cell-permeable anti-CRISPR protein (6xNLS-Acr) that efficiently inhibits Cas9 activity in human cells, enhancing genome-editing specificity.

This study uses MINFLUX nanoscopy to directly visualize MCM helicase activation and replisome coupling in human cells, showing sister replisomes remain coupled at ~40 nm during S phase.

WHaloForce is a chemigenetic tension sensor that images molecular forces in living cells and animals, revealing tissue-specific force patterns and ovulation-related molecular loading.

The paper shows DNA supercoiling selectively enhances productive homology search by RecA filaments in Caulobacter crescentus, independent of DNA compaction.

This study shows cohesin accelerates homology search in HR repair by transforming 3D diffusion into 1D scanning through loop extrusion and cohesion.

DNAsight automates AFM image analysis of chromatin organization, revealing protein-specific structural signatures and nucleosome spacing distributions.

The paper develops a statistical mechanics approach to identify sequence features controlling DNA cyclizability, finding periodic features in highly cyclizable sequences and distinct patterns in low cyclizability.

This paper introduces REVi-SCOPE, a platform that rapidly characterizes cardiac variants' functional impact, enabling precision cardiology by analyzing VUSs in 10 days.

This study uses single-molecule techniques to show that trigger factor interacts with nascent proteins via multiple weak, dynamic contacts that evolve during elongation.

A hybrid 4D whole-cell simulation of the minimal bacterium JCVI-syn3A integrates spatial reaction-diffusion, metabolism, polymer chromosome dynamics, and division across a complete 105-minute cell cycle.

This study optimizes single molecule assays to detect and quantify the native hair cell mechanosensory transduction complex at attomole levels.

The paper shows cohesin-driven chromatin loop extrusion facilitates homology search during RAD51-mediated DNA repair in mammalian cells.

The paper shows CTCF controls chromatin boundary permeability through a dynamic conformational ensemble, not static occupancy, influencing cohesin capture probabilities.

This study investigates the mechanisms of DNA and RNA strand transfer and protein exchange between SSB and RPA using smFRET, revealing dynamic interactions and length-dependent behavior.

The paper develops a single-molecule platform to study DNA supercoiling effects on protein-DNA interactions, finding that negative supercoiling increases Cas9 off-target effects and enhances MutS binding to mismatched bases.

This study shows that single nucleosomes encode A/B compartment information through biophysical properties, specifically condensability, which correlates with gene expression and is electrostatic in nature.

This study shows FoxP3 forms different supramolecular structures on microsatellites, enabling it to bridge DNA duplexes and coordinate genomic loci, with nucleosomes aiding assembly.

This paper develops a computational model to predict DNA mechanics from sequence, revealing mechanical selection in diverse eukaryotes and identifying extreme DNA mechanics in nature.

This study demonstrates that SHARP, a helicase-driven isothermal amplification method, effectively amplifies repetitive and compositionally extreme DNA sequences that are challenging for conventional PCR.

This study reveals how Rad52 sorts and stacks Rad51 at DNA junctions to enhance homologous recombination by promoting Rad51 binding to RPA-coated ssDNA.

This study investigates RPA's long-distance migration on ssDNA using biophysical methods, finding that intersegmental transfer via multivalent interactions enables rapid diffusion under tension.

This paper introduces sgGOLDFISH, a method for single-nucleotide sensitivity in direct hybridization genome imaging, and demonstrates its application in identifying and localizing point mutations in progeroid fibroblasts.

The paper tracks single mRNA translation in live cells, revealing that translation occurs in bursts and is influenced by 5' UTR structure and environmental signals.

This study identifies that pathological α-synuclein destabilizes the TSC1-TSC2 complex, activates mTORC1, and enhances protein translation, contributing to neurodegeneration in Parkinson's disease.