James M. Berger

This study shows SF1 helicase Rep translocates processively on ssDNA and, unexpectedly, also on dsDNA, with high speed and processivity, challenging existing models of helicase function.

This paper identifies triptolide as a compound that enhances the effectiveness of DNMTi by inhibiting DCTPP1, overcoming resistance in cancer cells.

This study shows that ORC interacts with HP1a via specific motifs to regulate heterochromatin structure and function in Drosophila.

DNAsight automates AFM image analysis of chromatin organization, revealing protein-specific structural signatures and nucleosome spacing distributions.

This study shows that chromatin buffers torsional stress during transcription by enabling Pol II to transcribe through nucleosomes more efficiently than on single-nucleosome substrates.

This study develops a high-resolution assay to show that the replisome, composed of helicase and DNA polymerase, is a powerful torsional motor that regulates DNA replication stalling and reactivation.

This study investigates the mechanisms of DNA and RNA strand transfer and protein exchange between SSB and RPA using smFRET, revealing dynamic interactions and length-dependent behavior.

The paper develops a single-molecule platform to study DNA supercoiling effects on protein-DNA interactions, finding that negative supercoiling increases Cas9 off-target effects and enhances MutS binding to mismatched bases.

This study demonstrates that SHARP, a helicase-driven isothermal amplification method, effectively amplifies repetitive and compositionally extreme DNA sequences that are challenging for conventional PCR.

This study identifies a new role for human topoisomerase IIα in promoting chromatin condensation through a polymer-collapse phase transition, independent of its catalytic activity.

This study characterizes the structure and function of the Spo11 core complex in S. cerevisiae, revealing its monomeric structure and DNA-binding properties related to meiotic DSB formation.

This study identifies a yeast Top2 mutant that generates stabilized cleavage intermediates, leading to de novo duplications via nonhomologous end-joining.