Author

B. M. Rao

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Biochemistry· 4 Bioengineering· 3 Cell Biology· 2

Signature Assays, Organisms & Methods

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Approach:experimental assay· 9 Assay:proteomics· 7 Research Focus:protein folding & dynamics· 5 Organism:yeast· 5 Research Focus:cell signaling & trafficking· 3 Research Focus:cell type annotation· 2 Research Focus:cell-cell communication· 1 Research Focus:chromatin & epigenetic regulation· 1

Frequent Co-Authors

S. Menegatti· 5 shared J. D. Bowen· 3 shared A. Blain· 2 shared G. T. Reeves· 2 shared K. Bacon· 2 shared M. Burroughs· 2 shared N. McArthur· 2 shared R. Das· 2 shared

Activity by Year

2019-2022 · peak 5/yr

Co-Author Collaboration Network

10 top collaborators · 18 mutual co-author links

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5 shared papersBiochemistry

S. Menegatti

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Author profile →

9 papers

Recent·Most cited

Experimental and analytical framework for ''mix-and-read'' assays based on split luciferase

N. McArthur, C. Cruz-Teran, A. Thatavarty, G. T. Reeves, B. M. Rao

This paper develops a 'mix-and-read' assay using split luciferase for sensitive detection and quantification of analytes in solution, with a model to predict assay performance.

bioRxiv · Feb 13, 2022Bioengineering

Identifying challenges and opportunities for histone peptides in protein engineering platforms

J. N. Meanor, A. J. Keung, B. M. Rao

This study identifies challenges in using histone peptides in protein engineering platforms and shows that manipulating binding avidity can improve specificity.

bioRxiv · Dec 28, 2021 · PreprintBioengineering

Use of target-displaying magnetized yeast in screening mRNA display peptide libraries to identify ligands

J. D. Bowen, K. B. Bacon, H. R. Reese, B. M. Rao, S. Menegatti

This paper uses magnetized yeast displaying protein targets to screen mRNA display peptide libraries and identifies ligands with measured binding affinities.

bioRxiv · Aug 13, 2020Biochemistry

Quantitative yeast-yeast two hybrid for discovery and binding affinity estimation of protein-protein interactions

K. Bacon, A. Blain, J. Bowen, M. Burroughs, N. McArthur, S. Menegatti, B. M. Rao

This paper introduces a quantitative yeast-yeast two hybrid system to discover and estimate binding affinities of protein-protein interactions.

bioRxiv · Aug 12, 2020Biochemistry

Screening of yeast display libraries of enzymatically-cyclized peptides to discover macrocyclic peptide ligands

J. D. Bowen, J. D. Schneible, C. R. Labar, S. Menegatti, B. M. Rao

The paper develops methods to cyclize peptides using transglutaminase in yeast display libraries and identifies cyclic peptides that bind YAP and its WW domains with high selectivity.

bioRxiv · Aug 8, 2020Biochemistry

Discovery of membrane-permeating cyclic peptides via mRNA display

J. D. Bowen, A. E. Schloop, G. T. Reeves, S. Menegatti, B. M. Rao

This paper identifies membrane-permeating cyclic peptides (MPPs) capable of crossing both cytoplasmic and outer embryonic membranes, outperforming linear counterparts in permeation.

bioRxiv · Jul 20, 2020Biochemistry

Genetically encoded live cell sensor for tyrosinated microtubules

S. Kesarwani, P. Lama, A. Chandra, P. P. Reddy, A. Jijumon, S. Bodakuntla, B. M. Rao, C. Janke, R. Das, M. Sirajuddin

The paper develops a genetically encoded live cell sensor for tyrosinated microtubules and uses it to study drug-induced microtubule depolymerization.

bioRxiv · Mar 29, 2020Cell Biology

Screening yeast display libraries against magnetized yeast cell targets enables efficient isolation of membrane protein binders.

K. Bacon, M. Burroughs, A. Blain, S. Menegatti, B. M. Rao

The paper demonstrates a method to isolate membrane protein binders using magnetized yeast cell targets, achieving efficient enrichment of low-frequency binders in a single screening round.

bioRxiv · Aug 7, 2019Bioengineering

A Fyn - specific biosensor reveals localized, pulsatile kinase activity and spatially regulated signaling crosstalk

A. Mukherjee, R. Singh, S. Udayan, S. Biswas, P. Pothula, S. Manmadhan, G. George, S. Dilip Kumar, R. Das, B. M. Rao, A. Gulyani

The paper develops a Fyn-specific biosensor to visualize localized, pulsatile kinase activity and spatially regulated signaling crosstalk in live cells.

bioRxiv · Aug 5, 2019 · PreprintCell Biology