S. Menegatti

Peptide ligands for the affinity purification of adeno-associated viruses from HEK 293 cell lysates

The paper develops peptide-based adsorbents to purify AAV2 from HEK 293 cell lysates, achieving high recovery and reduced host proteins with comparable transduction activity to commercial methods.

Design of 8-mer Peptides that Block Clostridioides difficile Toxin A in Intestinal Cells

The paper designs 8-mer peptides that block Clostridioides difficile toxin A in intestinal cells using a combination of computational and experimental methods.

In vivo genome-wide CRISPR screens identify SOCS1 as a major intrinsic checkpoint of CD4+ Th1 cell response

The paper identifies SOCS1 as a key intrinsic checkpoint limiting CD4+ Th1 cell response through in vivo CRISPR screens and shows targeting SOCS1 enhances antitumor T-cell therapy.

Use of target-displaying magnetized yeast in screening mRNA display peptide libraries to identify ligands

This paper uses magnetized yeast displaying protein targets to screen mRNA display peptide libraries and identifies ligands with measured binding affinities.

Quantitative yeast-yeast two hybrid for discovery and binding affinity estimation of protein-protein interactions

This paper introduces a quantitative yeast-yeast two hybrid system to discover and estimate binding affinities of protein-protein interactions.

Screening of yeast display libraries of enzymatically-cyclized peptides to discover macrocyclic peptide ligands

The paper develops methods to cyclize peptides using transglutaminase in yeast display libraries and identifies cyclic peptides that bind YAP and its WW domains with high selectivity.

Discovery of membrane-permeating cyclic peptides via mRNA display

This paper identifies membrane-permeating cyclic peptides (MPPs) capable of crossing both cytoplasmic and outer embryonic membranes, outperforming linear counterparts in permeation.

Screening yeast display libraries against magnetized yeast cell targets enables efficient isolation of membrane protein binders.

The paper demonstrates a method to isolate membrane protein binders using magnetized yeast cell targets, achieving efficient enrichment of low-frequency binders in a single screening round.