B. M. Woolston

Development of a recombineering system for the acetogen Eubacterium limosum with Cas9 counterselection for markerless genome engineering

This paper develops a recombineering system for Eubacterium limosum using RecT and Cas9 counterselection to enable markerless, scarless genomic modifications with high efficiency.

Prevotella are major contributors of sialidases in the human vaginal microbiome.

This study identifies Prevotella as a major source of sialidases in the vaginal microbiome, showing their prevalence and activity compared to Gardnerella.

Controlling pericellular oxygen tension in cell culture reveals distinct breast cancer responses to low oxygen tensions

This study shows that controlling incubator gas phase O2 is insufficient to control pericellular O2 in cell culture, revealing non-monotonic breast cancer responses to low O2.

Engineered bacteria titrate hydrogen sulfide and induce concentration-dependent effects on host in a gut microphysiological system

This study engineers E. coli to control H2S levels in a gut chip system, showing concentration-dependent effects on host gene expression and metabolism.

Identification and characterization of bacterial glycogen-degrading enzymes in the vaginal microbiome

This study identifies and characterizes bacterial glycogen-degrading enzymes in the vaginal microbiome, showing their activity and expression across different community states.

Cysteine dependence in Lactobacillus iners constitutes a novel therapeutic target to modify the vaginal microbiota

This study identifies cysteine dependence in Lactobacillus iners as a therapeutic target to shift vaginal microbiota from L. iners-dominant to L. crispatus-dominant states.