Efficient Homology-directed Repair with Circular ssDNA Donors
Sukanya Iyer, Aamir Mir, Joel Vega‐Badillo, Benjamin P. Roscoe, Raed Ibraheim, Lihua Julie Zhu, Jooyoung Lee, Pengpeng Liu, Kevin Luk, Esther Mintzer, Josias Soares de Brito, Phillip D. Zamore, Erik J. Sontheimer, Scot A. Wolfe
This paper demonstrates that circular ssDNA donors improve HDR efficiency and reduce off-target integration compared to linear ssDNA donors in mammalian cells.
bioRxiv · Dec 5, 2019 · PreprintMolecular Biology