Phillip D. Zamore

The paper identifies and characterizes miRNA binding sites in Drosophila using RNA Bind-n-Seq, revealing differences in binding site diversity and affinity compared to mammals.

This study identifies the essential role of Integrator, NEXT, and nuclear exosome complexes in Drosophila oogenesis by showing their necessity for RNA degradation and preventing aberrant RNA accumulation.

The paper shows that Piwi and piRNAs repress transcription of defective rRNA genes with truncated retrotransposon insertions in Drosophila ovaries, preventing aberrant rRNA accumulation.

This study shows pachytene piRNAs predominantly cleave hundreds of transcripts but only significantly alter the steady-state abundance of a minority, suggesting their evolutionary retention is driven by a few critical targets.

The paper shows that Drosophila males produce Su(Ste) piRNAs through 5'-to-3' phased biogenesis triggered by maternally deposited piRNAs, and this process is bypassed when mothers are XXY.

The paper reports cryo-EM structures of Drosophila Dicer-1 and Loqs-PB complex during pre-miRNA processing, revealing the mechanism of miRNA biogenesis.

This study shows PIWI-clade Argonaute proteins can cleave transposon transcripts with mismatches, making them more effective against mutating transposons than AGO-clade Argonautes.

This study shows that terminal modifications like 2'-O-methylation and trimming protect piRNAs from decay, while complementarity affects miRNA stability in mice and flies.

The paper shows that A-MYB activates TCFL5, which is essential for meiosis and spermiogenesis in placental mammals, and its loss causes sterility.

This study identifies a conserved piRNA locus, pi6, essential for male mouse fertility and embryo viability by regulating sperm function through mRNA cleavage.

This paper demonstrates that circular ssDNA donors improve HDR efficiency and reduce off-target integration compared to linear ssDNA donors in mammalian cells.

This study identifies Armitage as a protein linking piRNA amplification in nuage to phased piRNA production on mitochondria in Drosophila.

The paper introduces an automated Bayesian pipeline for analyzing single-molecule binding data and benchmarks it using TtAgo binding properties.

The paper shows Maelstrom represses canonical transcription in piRNA clusters, enabling Rhi to initiate piRNA precursor transcription.

The paper proposes a unified mechanism for piRNA biogenesis initiated by PIWI proteins, showing it operates across diverse animal species.

The paper develops methods to eliminate PCR duplicates in RNA-seq and small RNA-seq using unique molecular identifiers, showing improved data reproducibility and reduced bias.

The paper reports the genome of Trichoplusia ni, identifies small RNA pathways, and establishes genome editing protocols for Hi5 cells.