Author

Kevin Luk

University of Massachusetts Chan Medical SchoolORCID 0000-0002-9727-1960Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Genetics· 2 Molecular Biology· 2 Biochemistry· 1

Signature Assays, Organisms & Methods

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Approach:experimental assay· 4 Organism:human· 4 Assay:CRISPR screen· 3 Research Focus:variant calling· 3 Research Focus:gene regulatory network inference· 2 Research Focus:benchmarking· 1 Approach:deep learning· 1 Research Focus:drug response / drug discovery· 1

Frequent Co-Authors

Scot A. WolfeiD· 5 shared Pengpeng LiuiD· 3 shared Aamir MiriD· 2 shared Daniel E. BaueriD· 2 shared Erik J. SontheimeriD· 2 shared Karthikeyan PonnienselvaniD· 2 shared Lihua Julie ZhuiD· 2 shared Stacy A. Maitland· 2 shared

Activity by Year

2019-2026 · peak 2/yr

Co-Author Collaboration Network

10 top collaborators · 39 mutual co-author links

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5 shared papersGenetics

Scot A. WolfeORCID

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Author profile →

5 papers

Recent·Most cited

Fully Modified SpyCas9 Guide RNAs Enable Robust Genome Editing In Cells and In Vivo

Kim Anh Vu, Han Zhang, Nadia Amrani, Gitali Devi, Nicholas Gaston, Jonathan Lee, Stacy A. Maitland, Zexiang Chen, Dimas Echeverria, Pengpeng Liu, Karthikeyan Ponnienselvan, Matthew B. Hanlon, Connor Lucas, Kevin Luk, Jacquelyn Sousa, , Jonathan K. Watts

The paper develops fully modified SpyCas9 guide RNAs that maintain or enhance genome editing efficacy in vitro and in vivo.

bioRxiv · May 28, 2026 · PreprintBiochemistry

Generation of floxed alleles for cell-specific knockout in zebrafish

Masahiro Shin, Takayuki Nozaki, Benjamin J. Toles, Amy Kolb, Kevin Luk, Sumio Isogai, Kinji Ishida, Tomohito Hanasaka, Michael J. Parsons, Scot A. Wolfe, Nathan D. Lawson

The paper develops a protocol to generate floxed alleles in zebrafish for cell-specific knockout, demonstrating its utility in studying gene function.

bioRxiv · Sep 6, 2022 · PreprintGenetics

Optimization of NLS Composition Improves CRISPR-Cas12a Editing Rates in Human Primary Cells

Kevin Luk, Pengpeng Liu, Jing Zeng, Yetao Wang, Stacy A. Maitland, Feston Idrizi, Karthikeyan Ponnienselvan, Lihua Julie Zhu, Jeremy Luban, Daniel E. Bauer, Scot A. Wolfe

This study optimizes the nuclear localization signal (NLS) of Cas12a to enhance editing efficiency in human primary cells without compromising specificity.

bioRxiv · Feb 1, 2022 · PreprintMolecular Biology

ZNF410 represses fetal globin by devoted control of CHD4/NuRD

Divya S. Vinjamur, Qiuming Yao, Mitchel A. Cole, Connor McGuckin, Chunyan Ren, Jing Zeng, Mir Hossain, Kevin Luk, Scot A. Wolfe, Luca Pinello, Daniel E. Bauer

This study identifies ZNF410 as a novel repressor of fetal globin by targeting the NuRD complex, offering a new therapeutic target for beta-hemoglobinopathies.

bioRxiv · Aug 31, 2020 · PreprintGenetics

Efficient Homology-directed Repair with Circular ssDNA Donors

Sukanya Iyer, Aamir Mir, Joel Vega‐Badillo, Benjamin P. Roscoe, Raed Ibraheim, Lihua Julie Zhu, Jooyoung Lee, Pengpeng Liu, Kevin Luk, Esther Mintzer, Josias Soares de Brito, Phillip D. Zamore, Erik J. Sontheimer, Scot A. Wolfe

This paper demonstrates that circular ssDNA donors improve HDR efficiency and reduce off-target integration compared to linear ssDNA donors in mammalian cells.

bioRxiv · Dec 5, 2019 · PreprintMolecular Biology