REWRITE Enables Locus-Scale Engineering of Synthetic HLA Haplotypes in Human Pluripotent Stem Cells
Serena Generoso, Sarah Levovitz, Susanna Jaramillo, Minjoo Kim, Sumanth Dara, Shean Fu Phen, Bryan Yi, Tomoki Yanagi, Thomas L. DesMarais, Neta Agmon, Megan S. Hogan, LESLIE ANN MITCHELL, David M. Truong
REWRITE enables scar-minimized rewriting of native human loci exceeding 100 kb in hPSCs, demonstrated through synthetic refactoring of the class-I HLA region.
bioRxiv · Sep 16, 2025 · PreprintSynthetic Biology