David M. Truong

REWRITE Enables Locus-Scale Engineering of Synthetic HLA Haplotypes in Human Pluripotent Stem Cells

REWRITE enables scar-minimized rewriting of native human loci exceeding 100 kb in hPSCs, demonstrated through synthetic refactoring of the class-I HLA region.

Termination sequence between an inducible promoter and ubiquitous chromatin opening element (UCOE) reduces gene expression leakage and silencing

The paper investigates how spacer sequences between a promoter and A2UCOE affect gene expression leakage and silencing in iPSCs, finding that an AT-rich poly-A terminator reduces leakage and enhances anti-silencing.

Human macroH2A1 drives nucleosome dephasing and genome instability in histone-humanized yeast

This study replaces human variant histones in yeast, showing macroH2A1 disrupts chromatin organization and causes genome instability.

DASH/Dam1 complex mutants stabilize ploidy by weakening kinetochore-microtubule attachments

The paper shows that missense mutations in kinetochore proteins reduce chromosome instability in histone-humanized yeast by weakening microtubule attachments, promoting euploidy.

Comprehensive scanning mutagenesis of human retrotransposon LINE-1 identifies motifs essential for function

The paper identifies functionally important regions in ORF1p and ORF2p of human LINE-1 through trialanine mutagenesis, showing most mutants lose retrotransposition activity.

Superloser: a plasmid shuffling vector for Saccharomyces cerevisiae with exceedingly low background

The paper introduces Superloser, a plasmid shuffling vector for yeast that enables efficient gene pathway replacement with low background.

Resetting the yeast epigenome with human nucleosomes

This paper introduces human nucleosomes into yeast, showing adaptation through mutations and histone residue changes, revealing chromatin dynamics and transcriptional effects.