Megan S. Hogan

REWRITE enables scar-minimized rewriting of native human loci exceeding 100 kb in hPSCs, demonstrated through synthetic refactoring of the class-I HLA region.

The paper investigates how genomic context affects enhancer function by analyzing the murine Igf2/H19 locus and shows that regulatory elements depend on their native surroundings.

The paper uses synthetic regulatory genomics to study Sox2 expression in mESCs, finding that some enhancer regions are context-dependent and can enhance core regulatory elements.

The paper investigates how genomic context affects CTCF insulator function by analyzing reporter activity across various genomic configurations.

The paper introduces the Effector Index, an algorithm to predict causal genes at GWAS loci using genomic features, achieving 85% AUC in type 2 diabetes validation.

The paper quantifies how tissue context influences the effect of regulatory DNA variants on transcription factor activity using allelic accessibility maps in mice.

The paper describes a pipeline for de novo assembly, delivery, and expression of a 101 kb human gene in mouse cells, demonstrating functional evaluation of a full-length gene.