JACUZI-SD: An automated, high-throughput, minimally stressful approach to sleep depriving larval zebrafish.
Leah J. Elias, Harrison Khoo, François Kroll, Caroline Zhang, Soojung C. Hur, Jason Rihel, Seth Blackshaw
JACUZI-SD is a method to induce sleep deprivation in larval zebrafish with minimal stress, reducing total sleep time by 41-64% and eliciting rebound sleep.
iScience · May 1, 2026Peer-reviewedNeuroscience