Anti-CRISPR-mediated continuous directed evolution of CRISPR-Cas9 in human cells
Andrew L. Sabol, Amanuella A. Mengiste, Prashant Singh, Vedagopuram Sreekanth, Samuel J. Hendel, Minh Thuan Nguyen Tran, Anton M. Barybin, Santosh Kumar Chaudhary, Ra’Mal M. Harris, Kristi E. Liivak, Zachary C. Severance, Cale M. Locicero, Karishma Kailass, Chaiheon Lee, Xu Li, , Matthew D. Shoulders
This paper develops CRISPR-MACE, a platform for continuous directed evolution of CRISPR-Cas9 in human cells, generating variants with enhanced DNA binding and resistance to AcrIIA4.
Proceedings of the National Academy of Sciences · May 26, 2026Peer-reviewedSynthetic Biology