Amit Choudhary

This paper develops a cell-permeable anti-CRISPR protein (6xNLS-Acr) that efficiently inhibits Cas9 activity in human cells, enhancing genome-editing specificity.

This paper develops CRISPR-MACE, a platform for continuous directed evolution of CRISPR-Cas9 in human cells, generating variants with enhanced DNA binding and resistance to AcrIIA4.

This paper develops a small molecule, BRD8833, that induces BCR-ABL phosphorylation to disrupt its active site electric field, leading to loss of hyperactivity and apoptosis in BCR-ABL-dependent cancer cells.

This paper introduces GRIPs, a scalable platform for PTM editing using chimeric molecules, demonstrating functionality in multiple systems and confirming specificity through proteomics.

This paper develops a protein-based delivery system for anti-CRISPR proteins to precisely control Cas9 activity, reducing off-target effects and improving genome editing specificity.

This paper introduces an AMPK PHICS platform that enables controlled protein phosphorylation under physiological conditions, demonstrating its utility in regulating oncogenic signaling and phase separation.

This study shows induced-proximity inhibitors have orthogonal resistance mechanisms to classical inhibitors, using KRAS as a model system.

The paper introduces two ultrasmall chemogenetic tags, mgTag and cTag, which enable group-transfer reactions to append moieties to proteins, demonstrating their utility in cell signaling and proximity induction.

This study identifies small molecule inhibitors of TOX protein that reverse T cell exhaustion and show cytotoxicity in T-ALL.

This paper introduces a CRISPR-Cas9 conjugation platform for multivalent display of molecules, enhancing genome editing precision and enabling β-cell engineering to secrete interleukin-10.

The paper describes a gene-drive system in Drosophila controlled by an engineered Cas9 and a synthetic small molecule to enable chemical control of inheritance.