Uncovering synaptic and cellular nanoarchitecture of brain tissue via seamless in situ trimming and milling for cryo-electron tomography
Jiying Ning, Jill R. Glausier, Rangana Warshamanage, Leslie Gunther-Cummins, B. Tom Burnley, Colin M. Palmer, Guillermo González‐Burgos, Takeaki Miyamae, Jing Wang, Diane L. Carlisle, Chyongere Hsieh, Thomas Schmelzer, Silas A. Buck, Jonathan M. Franks, Cheri M. Hampton, , Zachary Freyberg
This paper introduces SISTM to map 3D synaptic nanoarchitecture in brain tissue across species using cryo-ET and STA, revealing conserved structural features in human postmortem tissue.
bioRxiv · Mar 9, 2025 · PreprintCell Biology