Zachary Freyberg

The paper uses AI to analyze pancreatic islet composition in type 1 diabetes, finding age, sex, and region-dependent changes and beta-cell loss with reciprocal cell expansion.

The paper identifies Tshz1 neurons in the nucleus accumbens as critical for withdrawal aversion by modulating dopamine release and mGluR8 activity, offering a new therapeutic target for opioid use disorder.

This study maps morphine-induced brain activity in male and female mice over time, revealing sex-specific and time-dependent patterns of c-Fos expression.

TomoPicker is a macromolecule localization method for cryo-ET tomograms that achieves comparable performance to supervised methods with significantly fewer annotations.

The paper develops a computational pipeline to link nuclear morphology to mRNA expression in neurons across aging in mice and humans, revealing subtype-specific morphology differences and age-related interactions.

This paper uses volume electron microscopy to analyze 3D synaptic structures in postmortem human prefrontal cortex, showing consistency with experimental models and revealing complex synaptic features.

This paper introduces SISTM to map 3D synaptic nanoarchitecture in brain tissue across species using cryo-ET and STA, revealing conserved structural features in human postmortem tissue.

The paper shows aging reduces mRNA expression of dopamine and glutamate markers in midbrain neurons of mice and humans, but not protein levels or cell numbers.

This paper identifies distinct roles of dopamine D3 and D1 receptors in regulating motivation and reinforcement in the nucleus accumbens, showing compartmentalized signaling within the same cell type.

This study identifies sex-specific roles of NPAS2 in fentanyl reward and addiction mechanisms in mice and humans.

The paper develops pharmacological tools to selectively target central versus peripheral dopamine D2-like receptors and shows their distinct roles in treating dysglycemia.

The paper identifies Ribosome-Associated Vesicles (RAVs) as dynamic platforms that facilitate activity-dependent local translation in neurons by transporting ribosomes to distal processes.

This study identifies keratinocytes and PDGF-B/PDGFRβ signaling as key mediators of peripheral opioid tolerance in mice, showing that PDGF-B release from keratinocytes drives tolerance through activation of PDGFRβ in sensory neurons.

The paper uses single nuclei RNA-sequencing to identify striatal cell types in humans and rhesus macaques and finds molecular changes linked to opioid use disorder.

This study identifies VGLUT-mediated mechanisms protecting DA neurons from PD-related degeneration, revealing sex-specific effects and transcriptional regulators.

The paper characterizes striatal projection neuron heterogeneity in mice using single-nuclei multi-omics and spatial mapping, revealing distinct subtypes with unique receptor expression and disease risk contributions.

The paper identifies synaptic protein alterations and circadian rhythm disruptions in brain regions associated with opioid use disorder using proteomics.

The paper introduces a cryo-FIB workflow to image brain tissue via in situ cryo-EM, enabling visualization of subcellular structures in fresh, unfixed mouse and postmortem human brain tissue.

This study identifies transcriptional changes in opioid use disorder brains, linking neuroinflammation and synaptic remodeling through RNA-seq and integrative analyses.

The paper identifies VGLUT expression as a sex-dependent mediator of age-related dopamine neuron vulnerability in Parkinson's disease.