D. J. Needleman

Measuring and modeling the dynamics of mitotic error correction

The paper quantifies mitotic error correction dynamics in human cells and validates a model explaining error reduction and anaphase timing.

Dual mechanism of kinetochore microtubule detachment

This paper investigates how kinetochore microtubules detach by combining experimental measurements and modeling, revealing a dual mechanism involving NDC80 complex binding energetics and microtubule catastrophe.

Laser Ablation and Fluid Flows Show a Single Force Mechanism Governs Spindle Positioning

The paper develops two techniques to study subcellular forces and applies them to show cortical pulling forces govern centrosome-related behaviors in C. elegans embryos.

Metabolic state of human blastocysts measured by fluorescence lifetime imaging microscopy

This study uses FLIM to measure metabolic state in human blastocysts, finding metabolic differences related to time since fertilization and developmental stage, but not morphology.

A CTDNEP1-lipin 1-mTOR regulatory network restricts ER membrane biogenesis to enable chromosome motions necessary for mitotic fidelity

This study identifies a CTDNEP1-lipin 1-mTOR network that limits ER membrane biogenesis to enable proper chromosome movements and mitotic fidelity.

A coarse-grained NADH redox model enables inference of subcellular metabolic fluxes from fluorescence lifetime imaging

This paper develops a coarse-grained NADH redox model to infer subcellular mitochondrial metabolic fluxes from fluorescence lifetime imaging in mouse oocytes and human cells.

Measuring NDC80 binding reveals the molecular basis of tension-dependent kinetochore-microtubule attachments

The paper measures NDC80 binding to microtubules in living cells and finds it is regulated by centromere tension and phosphorylation of Hec1.

Cooperative Accumulation Of Dynein-Dynactin At Microtubule Minus-Ends Drives Microtubule Network Reorganization

This study identifies how dynein-dynactin complexes accumulate at microtubule minus-ends to drive MT sliding, clustering, aster formation, and network contraction during mitosis.