Author

D. J. Nieves

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Biophysics· 4 Immunology· 1

Signature Assays, Organisms & Methods

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Assay:imaging· 3 Research Focus:cell signaling & trafficking· 2 Research Focus:cell type annotation· 2 Approach:experimental assay· 2 Assay:fluorescence / live-cell microscopy· 2 Organism:human· 2 Approach:microscopy & imaging analysis· 2 Research Focus:benchmarking· 1

Frequent Co-Authors

G. Hilzenrat· 3 shared K. Gaus· 3 shared D. M. Owen· 2 shared Z. Yang· 2 shared D. Sage· 1 shared E. A. K. Cohen· 1 shared E. Pandzic· 1 shared F. Levet· 1 shared

Activity by Year

2018-2021 · peak 2/yr

Co-Author Collaboration Network

10 top collaborators · 19 mutual co-author links

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3 shared papersBiophysics

G. Hilzenrat

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Author profile →

5 papers

Recent·Most cited

A framework for evaluating the performance of SMLM cluster analysis algorithms

D. J. Nieves, J. A. Pike, F. Levet, J. Griffie, D. Sage, E. A. K. Cohen, J.-B. Sibarita, M. Heilemann, D. M. Owen

The paper evaluates SMLM cluster analysis algorithms using ARI and IoU metrics across simulated scenarios to establish a standard framework for future algorithm development.

bioRxiv · Jun 19, 2021Biophysics

Quantitative mapping of membrane nanoenvironments through single-molecule imaging of solvatochromic probes

D. J. Nieves, D. M. Owen

The paper maps biophysical environments in mammalian cell membranes using solvatochromic probes and single-molecule imaging with nanoscale resolution.

bioRxiv · Jul 19, 2020 · PreprintBiophysics

tagPAINT: covalent labelling of genetically encoded protein tags for DNA-PAINT imaging

D. J. Nieves, G. Hilzenrat, J. Tran, Z. Yang, H. H. MacRae, M. A. B. Baker, J. J. Gooding, K. Gaus

The paper introduces tagPAINT, a covalent labeling method for DNA-PAINT imaging using genetically encoded protein tags, and applies it to study T-cell receptor signaling proteins.

bioRxiv · Jul 29, 2019Biophysics

Stoichiometric quantification of spatially dense assemblies with qPAINT

M. Baker, D. J. Nieves, G. Hilzenrat, J. F. Berengut, K. Gaus, L. K. Lee

This paper demonstrates that qPAINT measurements remain robust even when target molecules are as close as 3 nm apart, showing no significant cross-reactivity in dense assemblies.

bioRxiv · Jan 21, 2019Biophysics

Conformational states control Lck switching between free and confined diffusion modes in T cells

G. Hilzenrat, E. Pandzic, Z. Yang, D. J. Nieves, J. Goyette, J. Rossy, K. Gaus

This study shows that Lck's conformational states determine its diffusive behavior in T cells, with open conformations leading to confined diffusion and closed conformations allowing free diffusion.

bioRxiv · Oct 18, 2018Immunology