D. Tegunov

Disulfide constrained Fabs overcome target size limitation for high-resolution single-particle cryo-EM

This paper introduces Rigid-Fabs, disulfide-constrained antibody fragments that enable high-resolution cryo-EM structures of small proteins by overcoming size limitations.

Visualizing translation dynamics at atomic detail inside a bacterial cell

This study uses cryo-electron tomography to visualize translation dynamics in Mycoplasma pneumoniae, revealing ribosome states and showing how antibiotics affect translation.

Mechanism of SARS-CoV-2 polymerase inhibition by remdesivir

This study elucidates how remdesivir inhibits SARS-CoV-2 RNA-dependent RNA polymerase by causing RNA synthesis stalling through a translocation barrier.

Multi-particle cryo-EM refinement with M visualizes ribosome-antibiotic complex at 3.7 A inside cells

The paper introduces a software tool, M, for multi-particle cryo-EM refinement that achieves 3.7 Å resolution of a ribosome-antibiotic complex inside bacterial cells.

Structure of replicating SARS-CoV-2 polymerase

This study presents the cryo-EM structure of the SARS-CoV-2 RdRp complex, revealing its mechanism of RNA replication and potential antiviral targets.

Charting the native architecture of thylakoid membranes with single-molecule precision

The paper uses cryo-electron tomography to map the native molecular landscape of thylakoid membranes in green algae, revealing segregation of photosystems I and II at membrane domain borders.

Real-time cryo-EM data pre-processing with Warp

Warp processes cryo-EM data in real-time during acquisition, improving resolution and automating pre-processing steps.

Using the Volta phase plate with defocus for cryo-EM single particle analysis

This paper extends the use of the Volta phase plate in cryo-EM by incorporating defocus to improve data acquisition and achieve high-resolution 3D reconstructions.