W. Baumeister

Autophagy preferentially degrades non-fibrillar polyQ aggregates

This study shows autophagosomes preferentially degrade non-fibrillar polyQ aggregates by interacting with p62, while amyloid fibrils evade clearance due to their non-deformable structure.

Combinatorial selective ER-phagy remodels the ER during neurogenesis

The paper quantifies ER proteome remodeling during neurogenesis using ER-phagy receptors and identifies receptor-specific ER protein clearance mechanisms.

Cryo-electron tomography suggests tubulin chaperones form a subset of microtubule lumenal particles with a role in maintaining neuronal microtubules

The paper uses cryo-ET to identify lumenal particles in neuronal microtubules, suggesting some are tubulin chaperones involved in maintaining microtubule structure during neuronal differentiation.

A high confidence Physcomitrium patens plasmodesmata proteome by iterative scoring and validation reveals diversification of cell wall proteins during evolution

The paper identifies a high-confidence plasmodesmata proteome in Physcomitrium patens using iterative scoring and validation, revealing evolutionary diversification of cell wall proteins.

In situ structural analysis reveals membrane shape transitions during autophagosome formation

This study uses cryo-electron tomography to analyze autophagosome formation in yeast, revealing membrane shape transitions and interactions during biogenesis.

The AAA+ chaperone VCP disaggregates Tau fibrils and generates aggregate seeds

This study shows that the AAA+ chaperone VCP disaggregates Tau fibrils and generates seeding-active Tau as a byproduct, suggesting a role in aggregate removal and spreading in tauopathies.

In situ architecture of the ciliary base reveals the stepwise assembly of IFT trains

The paper visualizes the native structure of the ciliary transition zone and the stepwise assembly of IFT trains in Chlamydomonas cells.

Determinants shaping the nanoscale architecture of the mouse rod outer segment

This study uses cryo-ET to reveal molecular connectors and supramolecular assemblies in mouse rod outer segments, explaining their structural organization and linking them to genetic diseases.

In situ architecture of neuronal alpha-Synuclein inclusions

This study uses cryo-ET to show that neuronal alpha-Synuclein inclusions contain fibrils intermixed with cellular membranes and are nucleated by oligomers.

Elasticity of dense actin networks produces nanonewton protrusive forces

The paper investigates how dense actin networks in podosomes generate protrusive forces by combining cryo-electron tomography and theoretical modeling.

PolyQ expansion does not alter the Huntingtin-HAP40 complex

This study shows that polyQ expansion in HTT does not change the structure of the HTT-HAP40 complex, as revealed by biophysical and cryo-EM analyses.

Molecular-scale visualization of sarcomere contraction within native cardiomyocytes

This study uses cryo-electron tomography to visualize sarcomere contraction in neonatal rat cardiomyocytes, revealing thin filament sliding and structural changes during muscle contraction.

Structural basis for VIPP1 oligomerization and maintenance of thylakoid membrane integrity

This study determines the structure of cyanobacterial VIPP1 rings using cryo-EM, revealing how VIPP1 oligomerization and lipid binding maintain thylakoid membrane integrity.

A selective autophagy pathway for phase separated endocytic protein deposits

The paper identifies Ede1 as a selective autophagy receptor for endocytic protein condensates, showing its role in phase separation and autophagic degradation.

Amyloid-like aggregates cause lysosomal defects in neurons via gain-of-function toxicity

This study shows that amyloid-like aggregates impair lysosomal function in neurons by sequestering AP-31, leading to neurotoxicity.

Charting the native architecture of thylakoid membranes with single-molecule precision

The paper uses cryo-electron tomography to map the native molecular landscape of thylakoid membranes in green algae, revealing segregation of photosystems I and II at membrane domain borders.

Template-free detection and classification of heterogeneous membrane-bound complexes in cryo-electron tomograms

The paper develops a template-free method to detect and classify membrane-bound complexes in cryo-ET data, revealing previously undetected ER translocon species and their structural and spatial characteristics.

Tricalbin-mediated contact sites control ER curvature to maintain plasma membrane integrity

The paper identifies Tcb proteins as mediators of ER curvature at ER-PM contact sites, facilitating lipid transport to maintain plasma membrane integrity under stress.

An integrated workflow for cross-linking/mass spectrometry

The paper introduces a workflow combining sequential digestion and Xi software to improve cross-linking mass spectrometry detection of protein interactions.

In situ architecture of the algal nuclear pore complex

This study reports the in situ architecture of the algal nuclear pore complex in Chlamydomonas reinhardtii and compares it to the human NPC, revealing structural variations between species.

Cryo-EM structure of haemoglobin at 3.2 A determined with the Volta phase plate

The paper determines the 3.2 Å structure of human haemoglobin using a Volta phase plate, demonstrating its applicability to smaller protein complexes than previously possible with conventional methods.

Using the Volta phase plate with defocus for cryo-EM single particle analysis

This paper extends the use of the Volta phase plate in cryo-EM by incorporating defocus to improve data acquisition and achieve high-resolution 3D reconstructions.