F. A. Heberle

Cryo-EM images of phase separated lipid bilayer vesicles analyzed with a machine learning approach

This paper develops a machine learning approach to identify phase-separated lipid domains in cryo-EM images with high accuracy.

Cell Membranes Sustain Phospholipid Imbalance Via Cholesterol Asymmetry

The paper reveals that mammalian plasma membranes have phospholipid imbalances due to cholesterol asymmetry, affecting membrane permeability and protein localization.

Rapid Synthesis of Cryo-ET Data for Training Deep Learning Models

This paper introduces a method to generate synthetic Cryo-ET data for training deep learning models, enabling accurate restoration and segmentation of real tomograms.

Partitioning to ordered membrane domains regulates the kinetics of secretory traffic

The paper investigates how membrane domains influence secretory traffic kinetics, finding that raft affinity affects Golgi exit rates but not ER exit.

Serinc5 restricts HIV membrane fusion by altering lipid order and heterogeneity in the viral membrane

This study shows Serinc5 restricts HIV infection by altering viral membrane lipid order and heterogeneity, reducing fusion pore formation and infectivity.

Optimization of cryo-electron microscopy for quantitative analysis of lipid bilayers

This paper optimizes cryo-EM parameters and analysis methods to accurately measure bilayer thickness and detect lateral heterogeneity in lipid membranes.

Direct label-free imaging of nanodomains in biomimetic and biological membranes by cryogenic electron microscopy

This paper uses cryoEM to directly image nanodomains in biomimetic and biological membranes without extrinsic probes, revealing structural differences at sub-angstrom resolution.

On the mechanism of bilayer separation by extrusion; or, why your large unilamellar vesicles are not really unilamellar

This paper shows extruded vesicles are often multilamellar, not unilamellar, and identifies factors that influence lamellarity.

PS membrane asymmetry influences the folding and insertion of a transmembrane helix

This study investigates how PS membrane asymmetry affects pHLIP topology and insertion, finding that lipid asymmetry influences membrane protein behavior.

Gramicidin increases lipid flip-flop in symmetric and asymmetric lipid vesicles

This study shows gramicidin increases lipid flip-flop in both symmetric and asymmetric lipid vesicles through concentration-dependent mechanisms.