Michelle S. Frei

This paper introduces a chemigenetic biosensor, WHaloEKAR, that reports ERK activity through fluorescence lifetime changes, enabling spatially resolved and multiplexed measurements in cultured cells and live animals.

The paper introduces nanobody-HaloTag constructs for multiplexed immunofluorescence imaging, enabling up to eight targets with lifetime and spectral encoding in a single step.

The paper introduces AKAR6, a new suite of FRET-based PKA sensors with enhanced sensitivity and selectivity for detecting compartment-specific PKA activity in live cells.

This paper develops highly sensitive chemigenetic FRET-based biosensors for kinase and signaling activity with improved dynamic range and spectral properties.

The paper introduces far-red chemigenetic kinase activity biosensors enabling five-color multiplexing, 4D imaging, and super-resolution via STED microscopy.

The paper introduces WHaloCaMP, a near-infrared calcium indicator, and demonstrates its use in imaging calcium responses in various organisms.