I. M. Cheeseman

Co-Essentiality Analysis Identifies PRR12 as a Regulator of Cohesin and Genome Integrity

The paper identifies PRR12 as a cohesin regulator through co-essentiality analysis, showing its role in genome integrity and context-specific effects across species.

Functional genetics reveals modulators of anti-microtubule drug sensitivity

The paper identifies gene targets that modulate sensitivity to anti-microtubule drugs using CRISPR screens in human cells.

Binding Site Maturation Modulated by Molecular Density Underlies Ndc80 Binding to Kinetochore Receptor CENP-T

The paper shows that Ndc80 binding to CENP-T is enhanced by molecular density, with maturation of binding sites in clusters accelerating complex formation.

Higher-order assembly is a regulatory switch that promotes outer kinetochore recruitment

The paper shows that higher-order assembly of CENP-T promotes outer kinetochore recruitment by increasing local density, thereby restricting kinetochore formation to centromeres.

Dynamic cell cycle-dependent phosphorylation modulates CENP-L-CENP-N centromere recruitment

This study shows phosphorylation of CENP-L and CENP-N regulates their complex formation and localization during the cell cycle, affecting chromosome segregation.

The phenotypic landscape of essential human genes

The paper uses CRISPR screening and microscopy to analyze phenotypes of essential human genes, identifying co-functional genes and functional contributions to chromosome segregation.

Alternative Cdc20 translational isoforms bypass the spindle assembly checkpoint to control mitotic arrest duration

The paper shows that alternative Cdc20 isoforms control mitotic arrest duration by bypassing the SAC, affecting cancer cell behavior and drug sensitivity.

Separase cleaves the kinetochore protein Meikin to direct the meiosis I/II transition

This study shows that Separase cleaves Meikin to control the meiosis I/II transition by altering its function and promoting Rec8 cleavage.

Polarized Dishevelled dissolution and condensation drives embryonic axis specification in oocytes

The paper shows that Dishevelled dynamics in sea star oocytes drive embryonic axis specification through dissolution and condensation at the vegetal pole, independent of Wnt signaling.

Identification of a Golgi-localized altORF peptide reveals a minimal Golgi targeting motif

The paper identifies a minimal Golgi targeting motif through analysis of an altORF peptide from CENP-R, revealing a 10 amino acid sequence and a critical cysteine residue essential for Golgi localization.

Differential Requirements for the CENP-O Complex Reveal Parallel PLK1 Kinetochore Recruitment Pathways

The paper identifies parallel PLK1 recruitment pathways involving the CENP-O complex and BUB1 by analyzing cell line-specific essentiality.

Selective dephosphorylation by PP2A-B55 directs the meiosis I - meiosis II transition in oocytes

The paper identifies PP2A-B55's role in meiosis I to II transition through selective dephosphorylation of threonine residues in sea star oocytes.

Reconstitution of human kinetochore in mitotic cell extracts reveals permitted and restricted assembly steps

Alpha-satellite RNA transcripts are repressed by centromere-nucleolus associations

The paper shows alpha-satellite RNA is repressed by centromere-nucleolus associations and fluctuates during the cell cycle.

The AAA+ ATPase TorsinA polymerizes into hollow tubes with a helical periodicity of 8.5 subunits per turn

This study characterizes the oligomerization of TorsinA, revealing helical filaments with a 8.5 subunit per turn periodicity and membrane tubulation activity.