Author

J. de Rutte

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

Click to filter author papers

Bioengineering· 9

Signature Assays, Organisms & Methods

Click to filter · or open tag

Approach:experimental assay· 9 Research Focus:cell type annotation· 5 Organism:human· 5 Research Focus:differential expression· 2 Assay:scRNA-seq· 2 Research Focus:cell-cell communication· 1 Assay:CITE-seq· 1 Research Focus:clustering· 1

Frequent Co-Authors

D. Di Carlo· 8 shared D. Koo· 5 shared R. Dimatteo· 4 shared S. Lee· 4 shared K. Ha· 2 shared M. van Zee· 2 shared S. Udani· 2 shared A. Bertozzi· 1 shared

Activity by Year

2020-2023 · peak 3/yr

Co-Author Collaboration Network

10 top collaborators · 33 mutual co-author links

Click any collaborator node to inspect or filter shared papers

Focal author (center)Selected co-authorORCID co-authorUnlinked name

Curved chords = mutual co-authorship

8 shared papersBioengineering

D. Di Carlo

Mutual co-authors in cluster

Author profile →

9 papers

Recent·Most cited

Defining T cell receptor repertoires using nanovial-based affinity and functional screening

D. Koo, Z. Mao, R. Dimatteo, N. Tsubamoto, M. Noguchi, J. McLaughlin, W. Tran, S. Lee, D. Cheng, J. de Rutte, G. Burton Sojo, O. Witte, D. Di Carlo

This study uses nanovials to screen antigen-specific T cells based on affinity and function, identifying a more specific functional TCR repertoire than standard techniques.

bioRxiv · Jan 17, 2023 · PreprintBioengineering

Secretion encoded single-cell sequencing (SEC-seq) uncovers gene expression signatures associated with high VEGF-A secretion in mesenchymal stromal cells

S. Udani, J. Langerman, D. Koo, S. Baghdasarian, B. Cheng, S. Kang, C. Soemardy, J. de Rutte, K. Plath, D. Di Carlo

SEC-seq links cell secretions to transcriptomes, revealing VEGF-A secretion heterogeneity and a unique gene signature in MSCs with high secretion.

bioRxiv · Jan 7, 2023Bioengineering

SEC-seq: Association of molecular signatures with antibody secretion in thousands of single human plasma cells

R. Y.-H. Cheng, J. de Rutte, A. R. Ott, L. Bosler, W.-Y. Kuo, J. Liang, B. E. Hall, D. J. Rawlings, D. Di Carlo, R. G. James

The paper introduces SEC-seq, a method to link antibody secretion with surface markers and transcriptomes in single human plasma cells, revealing pathways associated with high IgG secretion.

bioRxiv · Aug 25, 2022Bioengineering

Sorting Single T cells based on Secreted Cytokines and Surface Markers using Hydrogel Nanovials

D. Koo, R. Dimatteo, S. Lee, J. de Rutte, D. Di Carlo

This paper develops a method to sort T cells based on cytokine secretion and surface markers using hydrogel nanovials, finding that polyfunctional cells are more common in CD4+ T cells and remain viable after sorting.

bioRxiv · Apr 28, 2022 · PreprintBioengineering

Scalable Fabrication of 3D Structured Microparticles Using Induced Phase Separation

S. Lee, J. de Rutte, R. Dimatteo, D. Koo, D. Di Carlo

The paper develops a scalable method to produce hydrogel microparticles with localized surface chemistry for cell interfacing, achieving high throughput and precise shape and functionalization.

bioRxiv · Jul 14, 2021Bioengineering

High-throughput selection of microalgae based on biomass accumulation rates in production environments using PicoShell Particles

M. van Zee, J. de Rutte, R. Rumyan, C. Williamson, T. Burnes, R. Radakovits, A. Sonico Eugenio, S. Badih, D.-H. Lee, M. Archang, D. Di Carlo

This paper introduces PicoShells to select microalgae based on biomass accumulation rates in production environments, showing an 8% faster biomass accumulation in selected Chlorella populations.

bioRxiv · Feb 3, 2021 · PreprintBioengineering

Selective and Improved Photoannealing of Microporous Annealed Particle (MAP) Scaffolds

B. N. Pfaff, L. J. Pruett, N. J. Cornell, J. De Rutte, D. DiCarlo, C. Highley, D. R. Griffin

The paper introduces a heterofunctional PEG macromer for selective photopolymerization in MAP scaffolds, enhancing therapeutic delivery and 3D printing without affecting cell viability.

bioRxiv · Oct 27, 2020Bioengineering

Monodisperse drops templated by 3D-structured microparticles

C.-Y. Wu, B. Wang, J. de Rutte, M. Ouyang, A. Joo, M. Jacobs, K. Ha, A. Bertozzi, D. Di Carlo

This paper demonstrates a method to create uniform sub-nanoliter compartments using 3D-structured microparticles, enabling stable monodisperse drops without specialized equipment.

bioRxiv · Mar 22, 2020Bioengineering

Massively parallel encapsulation of single cells with structured microparticles and secretion-based flow sorting

J. de Rutte, R. Dimatteo, M. M. Archang, M. van Zee, D. Koo, S. Lee, A. C. Sharrow, P. J. Krohl, M. P. Mellody, S. Zhu, J. Eichenbaum, M. Kizerwetter, S. Udani, K. Ha, A. L. Bertozzi, , D. Di Carlo

This paper introduces a method to sort single cells based on secreted products using microparticles, enabling high-throughput screening of cell function.

bioRxiv · Mar 9, 2020Bioengineering