D. Di Carlo

Flow zoometry of Drosophila

This paper introduces flow zoometry, a high-throughput screening method for Drosophila, enabling rapid, automated, and high-resolution analysis of whole animals.

Single-tier point-of-care serodiagnosis of Lyme disease

This paper develops a single-tier point-of-care serodiagnostic test for Lyme disease using synthetic peptides and machine learning, achieving sensitivity and specificity comparable to traditional two-tier lab tests.

Optimizing Cell Therapy by Sorting Cells with High Extracellular Vesicle Secretion

The paper develops a method to enrich cells with high extracellular vesicle secretion and shows improved therapeutic outcomes in a mouse model.

Amphiphilic particle-stabilized nanoliter droplet reactors with a multi-modal portable reader for distributive biomarker quantification

This paper develops a portable droplet reactor system for biomarker quantification in resource-limited settings, achieving low detection limits and comparable accuracy to ELISA.

Defining T cell receptor repertoires using nanovial-based affinity and functional screening

This study uses nanovials to screen antigen-specific T cells based on affinity and function, identifying a more specific functional TCR repertoire than standard techniques.

Secretion encoded single-cell sequencing (SEC-seq) uncovers gene expression signatures associated with high VEGF-A secretion in mesenchymal stromal cells

SEC-seq links cell secretions to transcriptomes, revealing VEGF-A secretion heterogeneity and a unique gene signature in MSCs with high secretion.

Numerical investigation of the dynamics of a rigid spherical particle in a vortical cross-slot flow at moderate inertia

This study examines how a rigid spherical particle's dynamics and trajectory are influenced by its entry position and size in a vortical cross-slot flow at moderate inertia.

Improved humoral immunity and protection against influenza virus infection with a 3D porous biomaterial vaccine

This paper develops a 3D porous biomaterial vaccine platform that enhances humoral immunity and protection against influenza virus by tuning physical and chemical properties.

SEC-seq: Association of molecular signatures with antibody secretion in thousands of single human plasma cells

The paper introduces SEC-seq, a method to link antibody secretion with surface markers and transcriptomes in single human plasma cells, revealing pathways associated with high IgG secretion.

Sorting Single T cells based on Secreted Cytokines and Surface Markers using Hydrogel Nanovials

This paper develops a method to sort T cells based on cytokine secretion and surface markers using hydrogel nanovials, finding that polyfunctional cells are more common in CD4+ T cells and remain viable after sorting.

Scalable Fabrication of 3D Structured Microparticles Using Induced Phase Separation

The paper develops a scalable method to produce hydrogel microparticles with localized surface chemistry for cell interfacing, achieving high throughput and precise shape and functionalization.

Counting of Enzymatically Amplified Affinity Reactions in Hydrogel Particle-Templated Drops

This paper presents a method to digitally count enzymatic reactions using hydrogel particle-templated drops, achieving femtomolar detection with standard equipment.

Focused Ultrasound Modulation of Hepatic Neural Plexus Restores Glucose Homeostasis in Diabetes

The paper demonstrates that focused ultrasound activation of the hepatic neural plexus restores glucose homeostasis in diabetes models across multiple species.

High-throughput selection of microalgae based on biomass accumulation rates in production environments using PicoShell Particles

This paper introduces PicoShells to select microalgae based on biomass accumulation rates in production environments, showing an 8% faster biomass accumulation in selected Chlorella populations.

Repeatability and reproducibility of the wzi High Resolution Melting-based clustering analysis for Klebsiella pneumoniae typing

This study evaluates the repeatability and reproducibility of a wzi HRM-based clustering method for K. pneumoniae typing across different instruments and operators.

Activating an adaptive immune response from a hydrogel scaffold imparts regenerative wound healing.

This study shows that D-peptide crosslinked hydrogels induce adaptive immune responses that promote regenerative wound healing despite faster degradation.

Monodisperse drops templated by 3D-structured microparticles

This paper demonstrates a method to create uniform sub-nanoliter compartments using 3D-structured microparticles, enabling stable monodisperse drops without specialized equipment.

Formation of uniform reaction volumes using concentric amphiphilic microparticles

The paper develops a method to create uniform microscale reaction compartments using amphiphilic microparticles, enabling scalable and multiplexed assays with high sensitivity.

Massively parallel encapsulation of single cells with structured microparticles and secretion-based flow sorting

This paper introduces a method to sort single cells based on secreted products using microparticles, enabling high-throughput screening of cell function.

DEEP LEARNING-ENABLED POINT-OF-CARE SENSING USING MULTIPLEXED PAPER-BASED SENSORS

This paper presents a deep learning framework for designing and quantifying point-of-care sensors, demonstrating a paper-based VFA for high-sensitivity hsCRP testing with competitive accuracy and hook-effect mitigation.