D. J. Rawlings

Monoclonal antibodies derived from B cells in subjects with cystic fibrosis reduce Pseudomonas aeruginosa burden in mice

The paper isolates PcrV-specific B cells from cystic fibrosis patients and generates monoclonal antibodies that reduce Pseudomonas aeruginosa burden in mice.

The IFIH1-A946T risk variant promotes diabetes in a sex-dependent manner

This study shows the IFIH1-A946T risk variant increases T1D risk in females but not males by enhancing IFN I signaling and altering immune cell profiles in NOD mice.

Activated PI3Kδ specifically perturbs mouse Treg homeostasis and function leading to immune dysregulation

This study shows that increased PI3Kδ activity in Tregs disrupts their function and homeostasis, leading to immune dysregulation and autoimmunity in mice.

Advancing Cell Therapies: Single-Cell Profiling, Generation, Expansion, and Gene Delivery in Rhesus Macaque Plasma B Cells

This study expands NHP B cells ex vivo, identifies unique plasma cell markers, and establishes AAV transduction conditions for engineered B cell therapies.

Human plasma cells engineered to secrete bispecifics drive effective in vivo leukemia killing

This paper engineers human plasma cells to secrete bispecific antibodies that kill leukemia cells in vitro and in vivo, showing efficacy against B-acute lymphoblastic leukemia and acute myeloid leukemia.

IL-4 downregulates BCL6 to promote memory B cell selection in germinal centers

This study shows IL-4 downregulates BCL6 in GC B cells to promote memory B cell selection and regulate affinity maturation.

SEC-seq: Association of molecular signatures with antibody secretion in thousands of single human plasma cells

The paper introduces SEC-seq, a method to link antibody secretion with surface markers and transcriptomes in single human plasma cells, revealing pathways associated with high IgG secretion.

PTPN22 R620W gene editing in T cells enhances low avidity TCR responses

This study edits the PTPN22 R620W variant in T cells to show it enhances low avidity TCR responses, contributing to autoimmunity risk.

Efficient methods for generation and expansion of, and gene delivery to Rhesus Macaque plasma B cells

The paper develops methods to expand and transduce rhesus macaque B cells for potential cell therapy applications.