J. Gaertig

DomainFit: Identification of Protein Domains in cryo-EM maps at Intermediate Resolution using AlphaFold2-predicted Models

DomainFit identifies protein domains in low-resolution cryo-EM maps using AlphaFold2 models, revealing new proteins in cellular complexes.

Effect of alpha-tubulin acetylation on the doublet microtubule structure

This study examines how alpha-tubulin acetylation affects doublet microtubule structure in Tetrahymena cilia using cryo-EM, MD, and mass spectrometry, finding small-scale structural changes and a link to phosphorylation.

Molecular architecture of the ciliary tip revealed by cryo-electron tomography

The paper uses cryo-electron tomography to reveal the molecular architecture of the ciliary tip in Tetrahymena thermophila, showing cross-linked microtubules and structural features of the central pair.

Remodeling and activation mechanisms of outer arm dyneins revealed by cryo-electron microscopy

This study uses cryo-electron microscopy to reveal the structure of outer dynein arms attached to microtubules and proposes a model for their remodeling and activation through molecular dynamics simulations.

Microtubule glycylation promotes basal body attachment to the cell cortex

This study identifies tubulin glycylation as essential for basal body microtubule attachment to the cell cortex, which is critical for ciliary array organization.

LF4/MOK and a CDK-related kinase regulate the number and length of cilia in Tetrahymena

This study identifies LF4/MOK and CDKR1 as regulators of cilium length and number in Tetrahymena, showing LF4A inhibits intraflagellar transport and CDKR1 modulates LF4A activity.

Tetrahymena RIB72A and RIB72B are Microtubule Inner Proteins in the ciliary doublet microtubules

The paper identifies RIB72A and RIB72B as Microtubule Inner Proteins in Tetrahymena ciliary doublet microtubules and shows their role in ciliary structure and motility.