K. H. Bui

DomainFit: Identification of Protein Domains in cryo-EM maps at Intermediate Resolution using AlphaFold2-predicted Models

DomainFit identifies protein domains in low-resolution cryo-EM maps using AlphaFold2 models, revealing new proteins in cellular complexes.

Effect of alpha-tubulin acetylation on the doublet microtubule structure

This study examines how alpha-tubulin acetylation affects doublet microtubule structure in Tetrahymena cilia using cryo-EM, MD, and mass spectrometry, finding small-scale structural changes and a link to phosphorylation.

Heterogeneity of radial spokes structural components and associated enzymes in Tetrahymena cilia

The paper identifies subtype variations in radial spokes of Tetrahymena cilia, revealing Rsp3 paralogs-dependent structures and novel associated enzymes involved in ADP/ATP regulation and phosphorylation.

Integrated modeling of the Nexin-dynein regulatory complex reveals its regulatory mechanism

The paper uses cryo-EM and integrative modeling to localize DRC subunits in the N-DRC of Tetrahymena thermophila and identifies interacting coiled-coil proteins.

Regulatory mechanisms of the dynein-2 motility by post-translational modification revealed by molecular dynamic simulation

This study uses molecular dynamics to show that poly-glutamylation inhibits dynein-2 motility and targets retrograde transport to the A-tubule.

Molecular architecture of the ciliary tip revealed by cryo-electron tomography

The paper uses cryo-electron tomography to reveal the molecular architecture of the ciliary tip in Tetrahymena thermophila, showing cross-linked microtubules and structural features of the central pair.

Native doublet microtubules from Tetrahymena thermophila reveal the importance of outer junction proteins

The paper uses cryo-EM to analyze native doublet microtubules in Tetrahymena thermophila, identifying MIPs and showing their role in cilia function through CFAP77 knockout.

Cryo-EM structure of the Agrobacterium tumefaciens T-pilus reveals the importance of positive charges in the lumen

The paper reports cryo-EM structures of Agrobacterium tumefaciens T-pilus and related N-pilus, showing positive charges in the lumen are functionally important.

Chloroplast-localized translation for protein targeting in Chlamydomonas reinhardtii

The paper identifies a chloroplast envelope domain where translation occurs, supporting co-translational protein targeting in Chlamydomonas reinhardtii.

Remodeling and activation mechanisms of outer arm dyneins revealed by cryo-electron microscopy

This study uses cryo-electron microscopy to reveal the structure of outer dynein arms attached to microtubules and proposes a model for their remodeling and activation through molecular dynamics simulations.

Local computational methods to improve the interpretability and analysis of cryo-EM maps

The paper introduces local computational methods to improve cryo-EM map interpretability by avoiding over-sharpening and providing local B-factors and occupancy maps.

The inner junction complex of the cilia is an interaction hub that involves tubulin post-translational modifications

The paper identifies new inner junction proteins in cilia and shows they form an interaction hub involving tubulin post-translational modifications.

Crystal structure of human PACRG in complex with MEIG1

The paper reports the crystal structure of human PACRG with MEIG1 and models its role in microtubule junctions, revealing interactions with tubulin and evolutionary insights.

Identification and mapping of central pair proteins by proteomic analysis

The paper identifies 37 novel central pair (CP) protein candidates through proteomic analysis of Chlamydomonas mutants and presents a more complete model of CP protein localization.

Tubulin Lattice in Cilia is in a Stressed Form Regulated by Microtubule Inner Proteins

This study presents the near-atomic structure of Tetrahymena doublet microtubules, showing that microtubule inner proteins stabilize the tubulin lattice and contribute to the intrinsic stress in cilia.