Systematic Transmission Electron Microscopy-Based Identification and 3D Reconstruction of Cellular Degradation Machinery
K. Neikirk, Z. Vue, P. Katti, B. I. Rodriguez, S. A. Omer, J. Shao, T. A. Christensen, E. Garza Lopez, A. Marshall, C. Palavicino-Maggio, J. Ponce, A. Alghanem, L. Vang, T. Barongan, H. Beasley, , A. Hinton
This paper describes methods to identify and distinguish subcellular structures involved in macroautophagy using TEM and 3D reconstruction, with applications to quantify degradation machinery under different conditions.
bioRxiv · Sep 26, 2021Biophysics