J. M. Hibberd

Single nuclei sequencing reveals C4 photosynthesis is based on rewiring of ancestral cell identity networks

The paper uses single nuclei sequencing to show C4 photosynthesis evolves through rewiring of cell identity networks from C3 plants.

A transcription factor module mediating C2 photosynthesis

The paper identifies a MYC-MYB transcription factor module responsible for GLDP expression in bundle sheath cells, linking it to the evolution of C2 photosynthesis from C3.

Synergistic control of chloroplast biogenesis by MYB-related and Golden2-like transcription factors

The paper identifies MYB-related transcription factors as regulators of chloroplast biogenesis in liverworts and Arabidopsis, showing they work with GLK factors to control chloroplast development.

Compartmentation of photosynthesis gene expression between mesophyll and bundle sheath cells of C4 maize is dependent on time of day

The paper investigates diurnal changes in photosynthesis gene expression in maize, identifying transcription factors that regulate C4 gene expression in mesophyll and bundle sheath cells during peak photosynthesis.

An optimised transformation protocol for Anthoceros agrestis and three more hornwort species

The paper describes an optimized genetic transformation protocol for Anthoceros agrestis and three additional hornwort species, resulting in higher efficiency and new tools for cell biology studies.

A rapid and robust leaf ablation method to visualize bundle sheath cell chloroplasts in C3 species

The paper introduces a leaf ablation method to phenotype bundle sheath cells in C3 species, showing differences in chloroplast parameters between rice and maize.

High light can alleviate chilling stress in maize

This study shows high light reduces chilling stress in maize by alleviating photoinhibition and stress pathways, contradicting previous reports.

A rapid method to quantify vein density in C4 plants using starch staining

The paper develops a high-throughput method to quantify vein density in C4 plants using starch staining and image analysis, finding variation in dicots but not in monocots.

Delayed exposure of rice to light partially phenocopies C4 bundle sheath characteristics by reducing cell length

The paper shows delayed light exposure in rice reduces bundle sheath cell length and increases leaf length, mimicking C4 characteristics by altering cell cycle exit.

Streamlined regulation of chloroplast development in the liverwort Marchantia polymorpha

The paper investigates chloroplast development regulation in liverwort Marchantia polymorpha, finding that GLK controls chloroplast biogenesis while GATA and SCL have no detectable roles.

Plasmodesmal connectivity in C4 Gynandropsis gynandra is induced by light and dependent on photosynthesis

This study shows that plasmodesmal connectivity between mesophyll and bundle sheath cells in C4 dicot Gynandropsis gynandra is induced by light and dependent on photosynthesis.

The genome of Gynandropsis gynandra provides insights into whole-genome duplications and the evolution of C4 photosynthesis in Cleomaceae

This study presents the genome of Gynandropsis gynandra, revealing whole-genome duplications and gene retention patterns related to C4 photosynthesis evolution in Cleomaceae.

Abscisic Acid modulates neighbor proximity-induced leaf hyponasty in Arabidopsis

This study shows ABA biosynthesis and signaling are required to promote leaf hyponasty in response to a drop in the red to far-red ratio.

Phytochrome B regulates reactive oxygen signaling during abiotic and biotic stress in plants

This study shows that phytochrome B regulates reactive oxygen species production during both abiotic and biotic stress in plants, working with RBOH proteins to control stress responses.

Distinct C4 Sub-Types and C3 Bundle Sheath Isolation In The Paniceae Grasses

The paper examines C4 acid decarboxylase diversity in Paniceae grasses, showing species-specific transcript and enzyme activity patterns and developing a C3 bundle sheath isolation method.

The bundle sheath of rice is conditioned to play an active role in water transport as well as sulfur assimilation and jasmonic acid synthesis

The paper identifies gene expression patterns in rice bundle sheath cells, showing specialization in water transport, sulfur assimilation, and jasmonic acid synthesis, with conservation across C3 species.

Targeted knockdown of ribulose-1, 5-bisphosphate carboxylase-oxygenase in rice mesophyll cells impact on photosynthesis and growth

This study knocks down Rubisco small subunit genes in rice mesophyll cells, reducing Rubisco protein content and CO2 assimilation, leading to decreased biomass and grain yield.

A bipartite transcription factor module controlling expression in the bundle sheath of Arabidopsis thaliana

The paper identifies cis-elements and transcription factors regulating gene expression in the bundle sheath of Arabidopsis thaliana, providing insights into C4 photosynthesis evolution.

Induction of C4 genes evolved through changes in cis allowing integration into ancestral C3 gene regulatory networks

The paper investigates how C4 gene expression is induced through cis-regulatory changes in Gynandropsis gynandra, showing similar induction patterns to C3 genes but with stronger expression.

A partial C4 photosynthetic biochemical pathway in rice

This paper introduces a partial C4 photosynthetic pathway in rice by expressing four C4 enzymes, showing increased 13C incorporation into malate and aspartate but limited flux into the Calvin-Benson cycle.

A role for neutral variation in the evolution of C4 photosynthesis

The paper shows natural variation in C4 enzyme expression in Arabidopsis and demonstrates that high C4 enzyme expression is neutral in rice, supporting the role of neutral variation in C4 photosynthesis evolution.

Genome-wide transcription factor binding in leaves from C3 and C4 grasses

The paper identifies genome-wide transcription factor binding in leaves of C3 and C4 grasses using DNaseI-SEQ, revealing conserved sites and shared mechanisms in C4 photosynthesis gene regulation.

Fluorescent reporters for functional analysis in rice leaves

This paper identifies five fluorescent proteins suitable for high-resolution live imaging in rice leaves and introduces a method for transient transformation.

An optimised protocol for isolation of RNA through laser capture microdissection of leaf material

The paper describes an optimized protocol for RNA isolation from leaf cells using laser capture microdissection, improving RNA quality by modifying embedding and sectioning methods.

Expression of SULTR2;2 in the Arabidopsis bundle sheath is mediated by a highly conserved positive regulator

The paper identifies a conserved promoter region in the SULTR2;2 gene that mediates bundle sheath-specific expression in Arabidopsis and related species.

Synergistic binding of bHLH transcription factors to the promoter of the maize NADP-ME gene used in C4 photosynthesis is based on an ancient code found in the ancestral C3 state

The paper identifies a conserved cis-element pair in the NADP-ME promoter of maize that evolved from an ancestral C3 regulatory code to control C4 photosynthesis.