J. van der Torre

Single-molecule visualization of twin-supercoiled domains generated during transcription

This study visualizes twin-supercoiled domains formed during transcription using single-molecule experiments, showing equal upstream and downstream supercoiling without requiring RNA transcript drag.

Dynamic ParB-DNA interactions initiate and maintain a partition condensate for bacterial chromosome segregation

This study investigates how ParB-DNA interactions form a dynamic partition condensate for bacterial chromosome segregation using in vitro experiments and simulations.

CTCF is a DNA-tension-dependent barrier to cohesin-mediated DNA loop extrusion

The paper shows CTCF blocks cohesin-mediated DNA loop extrusion and regulates its direction and loop size, revealing it as an active regulator rather than a passive barrier.

Extracting and characterizing protein-free megabasepair DNA for in vitro experiments

The paper presents a method to isolate megabasepair-long deproteinated DNA for in vitro studies, enabling the investigation of chromosome organization using long DNA substrates.

ParS-independent recruitment of the bacterial chromosome-partitioning protein ParB

This study shows ParB can recruit itself to DNA independently of parS, enabling long-distance chromosome segregation in bacteria.

SMC complexes can traverse physical roadblocks bigger than their ring size

The paper shows that SMC complexes can extrude DNA loops around large DNA-bound obstacles, suggesting a nontopological mechanism for loop extrusion.

Condensin-driven loop extrusion on supercoiled DNA

This study investigates how condensin-driven DNA loop extrusion is influenced by supercoiling using single-molecule imaging and atomic force microscopy.