S. Gruber

All eukaryotic SMC proteins induce a twist of -0.6 at each DNA-loop-extrusion step

The paper measures the twist induced by eukaryotic SMC proteins during DNA loop extrusion and finds all three SMC complexes induce a consistent -0.6 twist per step.

A conserved antigen induces respiratory Th17-mediated serotype-independent protection against pneumococcal superinfection

The paper identifies a conserved antigen, LafB, that provides serotype-independent protection against pneumococcal superinfection via Th17 cells, independent of antibodies.

Dynamic ParB-DNA interactions initiate and maintain a partition condensate for bacterial chromosome segregation

This study investigates how ParB-DNA interactions form a dynamic partition condensate for bacterial chromosome segregation using in vitro experiments and simulations.

VirB, a transcriptional activator of virulence in Shigella flexneri, uses CTP as a cofactor

This study identifies CTP as a cofactor for VirB, a transcriptional activator in Shigella flexneri, showing it binds CTP and promotes DNA clamp closure.

Direct observation of a crescent-shape chromosome in Bacillus subtilis

The paper visualizes the crescent shape of the Bacillus subtilis chromosome in live cells and identifies key proteins involved in its structure.

MCB-613 exploits a collateral sensitivity in drug-resistant EGFR-mutant non-small cell lung cancer through covalent inhibition of KEAP1

The paper identifies MCB-613 as a compound that targets KEAP1 in EGFR inhibitor-resistant NSCLC cells, exploiting its selective essentiality through covalent inhibition.

Effects of Variants of Concern Mutations on the Force-Stability of the SARS-CoV-2:ACE2 Interface and Virus Transmissibility

This study examines how mutations in SARS-CoV-2 variants affect the force-stability of the SARS-CoV-2:ACE2 interface and virus transmissibility.

DNA segment capture by Smc5/6 holo-complexes

The paper describes how Smc5/6 complexes load and capture DNA segments through a mechanism involving the Nse5/6 loader and kleisin neck gate.

Chromosome remodelling by SMC/Condensin in B. subtilis is regulated by Soj/ParA during growth and sporulation

The paper shows that ParA/Soj proteins regulate SMC complex redistribution during axial filament formation in B. subtilis, using monomeric ParA/Soj that cannot bind DNA or hydrolyse ATP.

The ParB clamp docks onto Smc for DNA loading via a joint-ParB interface

This study shows that ParB binds to the Smc complex in bacteria, forming a module that organizes and segregates chromosomes.

A Conformational Transition of Von Willebrand Factor's D'D3 Domain Primes It For Multimerization

This study uses single-molecule force spectroscopy to show that a conformational change in VWF's DD3 domain, triggered by pH changes and stabilized by FVIII, primes it for multimerization.

ParS-independent recruitment of the bacterial chromosome-partitioning protein ParB

This study shows ParB can recruit itself to DNA independently of parS, enabling long-distance chromosome segregation in bacteria.

A Tethered Ligand Assay to Probe SARS-CoV-2:ACE2 Interactions

This paper introduces a tethered ligand assay to study SARS-CoV-2:ACE2 interactions under mechanical force, showing higher stability and lower dissociation rates compared to SARS-CoV-1.

Spatio-temporal control of DNA replication by the pneumococcal cell cycle regulator CcrZ

This study identifies CcrZ as a regulator that couples DNA replication with cell division in pneumococci by controlling DnaA activity, ensuring proper chromosome inheritance.

Relief of ParB autoinhibition by parS DNA catalysis and ParB recycling by CTP hydrolysis promote bacterial centromere assembly.

This study investigates how CTP hydrolysis and parS DNA catalysis influence ParB clamp closure and recycling, contributing to bacterial centromere assembly.

DNA tension-modulated translocation and loop extrusion by SMC complexes revealed by molecular dynamics simulations

This study uses molecular dynamics simulations to show how DNA tension modulates SMC complex translocation and loop extrusion, revealing distinct behaviors under different tension conditions.

Nse5/6 inhibits the Smc5/6 ATPase to facilitate DNA substrate selection.

The paper shows Nse5/6 inhibits Smc5/6 ATPase activity, modulating DNA substrate selection in yeast.

Gradual opening of Smc arms in prokaryotic condensin

This study investigates the conformational changes in B. subtilis Smc-ScpAB, showing that arm separation depends on ATP and DNA, and that specific contacts regulate Smc function.

Fine-tuning of the Smc flux facilitates chromosome organization in B. subtilis

The paper identifies factors that influence bacterial chromosome organization by modulating Smc complex encounters and shows that these parameters are fine-tuned to reduce collisions.

A Tethered Ligand Assay to Probe the SARS-CoV-2 ACE2 Interaction under Constant Force

This paper introduces a tethered ligand assay to study SARS-CoV-2 ACE2 interactions under constant force and finds that the interaction is more mechanically stable and has a slower off-rate compared to SARS-CoV-1.

A rod conformation of the Pyrococcus furiosus Rad50 coiled coil

The paper presents a crystal structure of an alternate conformation of the Pyrococcus furiosus Rad50 coiled coil, showing co-aligned coils instead of the previously reported anti-parallel arrangement.

Designed Anchoring Geometries Determine Lifetimes of Biotin-Streptavidin Bonds under Constant Load and Enable Ultra-Stable Coupling

The paper investigates engineered streptavidin variants under constant force to determine how anchoring geometries affect biotin-streptavidin bond lifetimes, identifying a geometry that enables ultra-stable single molecule force spectroscopy.

Phospho-regulation of the Shugoshin - Condensin interaction at the centromere in budding yeast

The paper identifies a phosphorylation-regulated interaction between shugoshin and condensin at the centromere in budding yeast, essential for biorientation and chromosome segregation.

DNA-segment-capture model for loop extrusion by structural maintenance of chromosome (SMC) protein complexes

The paper introduces a DNA-segment-capture model for SMC protein loop extrusion, predicting tension-dependent translocation velocity and step size.

Separate Compartments for Chromosome Entrapment and DNA Binding during SMC translocation

The paper shows DNA binding occurs in the SMC compartment and chromosome entrapment in the Kleisin compartment of B. subtilis Smc/ScpAB, with DNA translocation blocked by mutations affecting DNA binding.

Temperature-dependent estimation of Gibbs energies using an updated group contribution method

The paper updates a group contribution method to estimate Gibbs energies and reaction equilibrium constants across temperatures and metal ion concentrations.