Steven D. Bruner

The paper introduces HARP, a yeast-based platform for discovering protease inhibitors, and demonstrates its ability to isolate low-nanomolar-range nanobodies against specific proteases.

This paper develops a yeast haploid model to study SRP54 mutations and their functional consequences, revealing dominant-negative phenotypes and growth defects associated with specific mutations.

This study improves the aerotolerance and activity of sulfide-dependent THI4 enzymes through directed evolution in yeast, identifying beneficial mutations that enhance their performance under aerobic conditions.

The paper describes a method to improve enzymes for plant applications using continuous directed evolution in yeast, demonstrating its effectiveness with a bacterial thiamin synthesis enzyme.

The paper identifies aerotolerant, multiple-turnover THI4 thiazole synthases in aerobic prokaryotes and shows they use cobalt as a cofactor instead of iron.