K. A. Breau

An in vitro platform for quantifying cell cycle phase lengths in primary human intestinal stem cells

The paper develops two fluorescent reporter systems and imaging platforms to analyze cell cycle phases in living primary human intestinal stem cells.

A new microphysiological system shows hypoxia primes human ISCs for interleukin-dependent rescue of stem cell activity

This study uses a microphysiological system to show hypoxia primes human intestinal stem cells (hISCs) to respond to interleukins, enhancing their activity under hypoxic conditions.

SOX9 elongates cell cycle phases and biases fate decisions in human intestinal stem cells

This study shows SOX9 in human intestinal stem cells slows proliferation, extends G1 phase, and biases differentiation toward specific cell fates.

A Leaky Human Colon Model Reveals Uncoupled Apical/Basal Cytotoxicity in Early Clostridioides difficile Toxin Exposure

This study develops a human colon model to assess how C. difficile toxins affect epithelial cells from different sides, finding that basal exposure causes more severe toxicity.

Transcriptomics informs design of a planar human enterocyte culture system that reveals metformin enhances fatty acid export

The paper develops a human enterocyte culture system to study lipid handling and finds metformin enhances fatty acid export by promoting fatty acid oxidation.

A proximal-to-distal survey of healthy adult human small intestine and colon epithelium by single-cell transcriptomics

The paper uses single-cell transcriptomics to analyze healthy adult human small intestine and colon epithelium, identifying lineage markers, regional differences, and functional variations across anatomical regions.