L. M. Oltrogge

A map of the rubisco biochemical landscape

The paper maps the sequence-to-function relationship of rubisco by assaying single amino acid mutants in E. coli, identifying conserved positions and mutations that improve CO2 affinity.

α-carboxysome size is controlled by the disordered scaffold protein CsoS2

This study identifies CsoS2 as the protein controlling α-carboxysome size through its interactions with the shell, proposing a model based on repeat classes and curvature preferences.

Autotrophic biofilms sustained by deeply-sourced groundwater host diverse CPR bacteria implicated in sulfur and hydrogen metabolism

The paper identifies CPR bacteria in sulfide-rich biofilms, linking them to sulfur and hydrogen metabolism through genomic and spectroscopic analyses.

Exploring masses and internal mass distributions of single carboxysomes in free solution using fluorescence and interferometric scattering in an anti-Brownian trap

Trajectories for the evolution of bacterial CO2-concentrating mechanisms

This paper studies the evolution of bacterial CO2-concentrating mechanisms by engineering E. coli to express ancestral forms of these systems and testing their growth under varying CO2 concentrations.

Ratiometric Sensing of Redox Environments Inside Individual Carboxysomes Trapped in Solution

The paper improves the ISABEL trap to monitor redox environments inside individual carboxysomes using ratiometric fluorescence.

Rubisco forms a lattice inside alpha-carboxysomes

The paper uses cryo electron tomography to image carboxysomes and reveals a novel lattice arrangement of Rubisco inside the microcompartment.

The encapsulin from Thermatoga maritima is a flavoprotein with a symmetry matched ferritin-like cargo protein

This study investigates the structure and function of the T. maritima encapsulin, revealing its role in cargo protein encapsulation and enzymatic activity through symmetry-matching and flavin binding.

Discovery and characterization of a novel family of prokaryotic nanocompartments involved in sulfur metabolism

The paper identifies a new prokaryotic nanocompartment in Synechococcus elongatus that enhances cysteine desulfurase activity during sulfate starvation.

α-carboxysome formation is mediated by the multivalent and disordered protein CsoS2

The paper identifies CsoS2's N-terminal peptide as a multivalent interaction hub that binds Rubisco through electrostatic interactions, enabling efficient α-carboxysome formation via avidity.