G. J. Jensen

Structure and Function of the Dot/Icm T4SS

This study constructs a nearly-complete model of the Legionella pneumophila Dot/Icm T4SS using subtomogram averaging and integrative modeling, revealing structural insights into six new components and their functional interactions.

Cryo-EM structure of the Agrobacterium tumefaciens type IV secretion system-associated T-pilus reveals stoichiometric protein-phospholipid assembly

The paper presents a cryo-EM structure of the Agrobacterium tumefaciens T-pilus, revealing a 1:1 stoichiometric assembly of VirB2 and phosphatidylglycerol with unique electrostatic interactions and structural features.

Structural remodeling of Coxiella burnetii during its biphasic developmental cycle revealedby cryo-electron tomography

This study uses cryo-electron tomography to reveal structural changes in Coxiella burnetii during its biphasic developmental cycle, showing morphological transitions and T4BSS-associated structures.

Geometric effects in gas vesicle buckling under ultrasound

This study investigates how the geometry of gas vesicles affects their buckling behavior under ultrasound, showing diameter has an inverse cubic effect on buckling pressure while length has minimal impact.

Dynamic structural adaptations enable the endobiotic predation of bdellovibrio bacteriovorus

This study uses cryo-electron tomography to image the predation cycle of Bdellovibrio bacteriovorus, revealing structural adaptations during prey invasion and replication.

In situ structure and organisation of the type IX secretion system

The paper uses cryo-electron tomography and proteomics to study the structure and organisation of the type IX secretion system in Bacteroidetes, identifying new interactions and proposing a model for substrate secretion.

In situ architecture of human kinetochore-microtubule interface visualized by cryo-electron tomography

The paper uses cryo-electron tomography to visualize the native architecture of the kinetochore-microtubule interface in human cells during mitosis, revealing distinct structural features.

Bimodally oriented cellulose fibers and reticulated homogalacturonan networks - A direct visualization of Allium cepa primary cell walls

This study visualizes the ultrastructure of onion cell walls, revealing bimodal cellulose fiber orientation and HG-rich meshing networks.

Rubisco forms a lattice inside alpha-carboxysomes

The paper uses cryo electron tomography to image carboxysomes and reveals a novel lattice arrangement of Rubisco inside the microcompartment.

Nanoscale details of mitochondrial fission revealed by cryo-electron tomography

This study uses cryo-electron tomography to reveal nanoscale details of mitochondrial fission in mammalian cells, showing coordinated membrane constriction and the role of actin and septin filaments.

Montage electron tomography of vitrified specimens

The paper introduces a montage electron tomography method to improve field of view while maintaining resolution in cryo-EM, demonstrating its effectiveness on HeLa cells.

A novel widespread bacterial structure related to the flagellar type III secretion system

The paper identifies a novel widespread bacterial structure related to the flagellar type III secretion system, suggesting its involvement in diverse cellular functions.

The stress-sensing domain of activated IRE1α forms helical filaments in narrow ER membrane tubes

This study determines the in-situ structure of IRE1 oligomers in ER tubes using cryo-CLEM, revealing helical filaments formed by IRE1 lumenal domain dimers and suggesting positive feedback in IRE1 signaling.

Measuring gas vesicle dimensions by electron microscopy

The paper explains discrepancies in gas vesicle diameter measurements and provides accurate methods using electron microscopy.

Challenges in solving structures from radiation-damaged tomograms of protein nanocrystals assessed by simulation

This paper evaluates a data processing pipeline for solving protein structures from radiation-damaged tomograms of nanocrystals using simulations.

Rapid Tilt-Series Method for Cryo-Electron Tomography: Characterizing Stage Behavior During FISE Acquisition

The paper characterizes the behavior of a single axis holder during FISE acquisition to balance data quality and speed in cryo-electron tomography.

Repurposing a macromolecular machine: Architecture and evolution of the F7 chemosensory system

The paper traces the evolutionary origin of the F7 chemosensory system in E. coli by analyzing ancestral forms in related bacteria and identifying structural and functional changes.

Structure of the bacterial cellulose ribbon and its assembly-guiding cytoskeleton by electron cryotomography

This study uses cryo-electron tomography to reveal the structure of bacterial cellulose ribbons and a novel cytoskeletal structure called the cortical belt in Gluconacetobacter species.

CryoEM structure of the Vibrio cholerae Type IV competence pilus secretin PilQ

The paper determines the cryoEM structure of V. cholerae PilQ, revealing its architecture and function in DNA transfer, and identifies it as a potential drug target.

A Prokaryotic Membrane Sculpting BAR Domain Protein

This study identifies BdpA, the first prokaryotic BAR domain protein, which regulates membrane curvature and OME formation in Shewanella oneidensis MR-1.

Plasma membrane damage removal by F-actin-mediated shedding from repurposed filopodia

The paper uses electron cryotomography to show that filopodia-like protrusions mediate plasma membrane damage repair through F-actin dynamics and ESCRT components.

Correlated cryogenic fluorescence microscopy and electron cryotomography shows that exogenous TRIM5α can form hexagonal lattices or autophagy aggregates in vivo

The paper uses cryo-CLEM to show TRIM5α forms hexagonal lattices and autophagy-related structures in mammalian cells.

Bacterial flagellar motor PL-ring disassembly sub-complexes are widespread and ancient

The paper shows that bacterial flagellar motor PL-ring sub-complexes persist after disassembly across multiple phyla and are ancient, not recently transferred.

Simulations of proposed mechanisms of FtsZ-driven cell constriction

This study simulates two models of FtsZ-driven bacterial cell constriction to determine which mechanisms can sustain constriction under turgor pressure.

Bacterial swarming reduces Proteus mirabilis and Vibrio parahaemolyticus cell stiffness and increases β-lactam susceptibility

In vivo structure of the Legionella type II secretion system by electron cryotomography

The paper presents the first in situ structure of the Legionella T2SS using cryo-ET, revealing architectural similarities and differences with T4P and supporting the piston model for substrate extrusion.

Fusion of DARPin to aldolase enables visualization of small protein by cryoEM

The paper describes a platform using DARPin fused to aldolase to visualize small proteins by cryoEM, achieving 5-8 Å resolution for GFP.

Rapid Tilt-Series Acquisition for Electron Cryotomography

The paper introduces two methods for faster tilt-series acquisition in electron cryotomography, with trade-offs in resolution and speed.

ETDB-Caltech: a blockchain-based distributed public database for electron tomography

The paper introduces a blockchain-based distributed database for storing and sharing electron tomography datasets, providing a user-friendly interface for data access and analysis.

Simulations suggest a constrictive force is required for Gram-negative bacterial cell division

The paper uses simulations to show that a constrictive force is needed for Gram-negative bacterial cell division, as cell wall remodeling alone is insufficient.