E. Nogales

Molecular interplay between HURP and Kif18A in mitotic spindle regulation

This study investigates how HURP regulates Kif18A motility and microtubule dynamics during mitosis using single-molecule imaging and Cryo-EM.

Structural basis for the inhibition of PRC2 by active transcription histone posttranslational modifications

The paper reveals how H3K4me3 and H3K36me3 inhibit PRC2 activity by blocking its interaction with chromatin and allosteric activators, using cryo-EM.

The activated plant NRC4 immune receptor forms a hexameric resistosome

The study determines the cryo-EM structure of the auto-active NRC4 resistosome, revealing a hexameric configuration that triggers immune responses via cytosolic Ca2+ influx.

Activation of automethylated PRC2 by dimerization on chromatin

The paper reveals a mechanism by which automethylated PRC2 dimerizes on chromatin to allosterically activate another PRC2 protomer for H3K27me3 methylation.

Structural and quantum chemical basis for OCP-mediated quenching of phycobilisomes

The paper reveals the structure of the OCP-PBS complex and elucidates the quantum mechanism of energy transfer during non-photochemical quenching in cyanobacteria.

Structures of the Cyanobacterial Phycobilisome

This paper presents cryo-EM structures of the cyanobacterial phycobilisome, revealing new conformations and regulatory mechanisms of light harvesting.

Structure of the Quenched Cyanobacterial OCP-Phycobilisome Complex

The paper reports the structure of the OCP-PBS complex in cyanobacteria at 2.7 Å resolution, revealing how it mediates photoprotective quenching.

CRISPR-Cas9 bends and twists DNA to read its sequence

This paper reveals how CRISPR-Cas9 bends and twists DNA to flip nucleotides toward guide RNA for sequence interrogation.

Molecular Organization of the Early Stages of Nucleosome Phase Separation Visualized by Cryo-Electron Tomography

This study uses cryo-electron tomography and deep learning to visualize the molecular organization of nucleosome phase separation stages, revealing a two-step process and the role of histone H1 in condensate transformation.

Structural transitions in the GTP cap visualized by cryo-EM of catalytically inactive microtubules

The paper uses cryo-EM to visualize the structure of GTP-bound microtubules, revealing an expanded lattice and closed seam that stabilize the GTP cap.

The encapsulin from Thermatoga maritima is a flavoprotein with a symmetry matched ferritin-like cargo protein

This study investigates the structure and function of the T. maritima encapsulin, revealing its role in cargo protein encapsulation and enzymatic activity through symmetry-matching and flavin binding.

Efficient graphene oxide coating improves cryo-EM sample preparation and data collection from tilted grids

This paper improves cryo-EM sample preparation by modifying graphene oxide coating to enhance particle density and reduce drift during tilted data collection.

Structure of the human SAGA coactivator complex

The paper presents the 2.9 Å structure of the human SAGA coactivator complex, revealing unique structural features and functional modules with implications for transcription and splicing in disease and cancer.

JARID2 and AEBP2 regulate PRC2 activity in the presence of H2A ubiquitination or other histone modifications

This paper reports the cryo-EM structure of human PRC2 with cofactors JARID2 and AEBP2 bound to a nucleosome containing H2AK119ub1, revealing their roles in PRC2 activity regulation and histone modification crosstalk.

The mechanism of motor inhibition by microtubule-associated proteins

This study investigates how microtubule-associated proteins (MAPs) regulate motor protein interactions, finding that MAPs inhibit motors without blocking their binding sites and that motors must interact with MAPs to move on MAP-coated microtubules.

Structure of the activated Roq1 resistosome directly recognizing the pathogen effector XopQ

The paper presents the cryo-EM structure of the activated Roq1 NLR bound to XopQ, revealing direct effector recognition and resistosome activation mechanism.

Discovery and characterization of a novel family of prokaryotic nanocompartments involved in sulfur metabolism

The paper identifies a new prokaryotic nanocompartment in Synechococcus elongatus that enhances cysteine desulfurase activity during sulfate starvation.

The cryo-electron microscopy structure of the human CDK-activating kinase

This paper determines the cryo-EM structure of human CAK, revealing its architecture and inhibitor binding, which informs therapeutic design.

Effects of α-tubulin acetylation on microtubule structure and stability

This study uses cryo-EM and molecular dynamics to show that α-tubulin acetylation at K40 reduces loop disorder and may affect microtubule stability.

The complete structure of the human TFIIH core complex

The paper presents the complete structure of the human TFIIH core complex using cryo-electron microscopy, revealing assembly mechanisms and disease mutation sites.

Temperature-responsive competitive inhibition of CRISPR-Cas9

This study shows AcrIIA2 and AcrIIA2b inhibit Cas9 DNA binding through structural occlusion, with temperature-dependent effects due to structural and environmental differences.

Visualization of PRC2-Dinucleosome Interactions Leading to Epigenetic Repression

The paper visualizes how PRC2 interacts with dinucleosomes to mediate epigenetic repression through cryo-EM structures.

Disabling Cas9 by an anti-CRISPR DNA mimic

This study shows that AcrIIA4 inhibits Cas9 by binding to Cas9-sgRNA complexes, reducing off-target edits while allowing on-target gene editing in human cells.