W. E. Moerner

Exploring transient states of PAmKate to enable improved cryogenic single-molecule imaging

This study investigates the photophysical properties of PAmKate to enhance cryogenic single-molecule imaging by improving emitter localization rates through a 2-color bleaching process.

Nanoscale cellular organization of viral RNA and proteins in SARS-CoV-2 replication organelles

This study uses super-resolution microscopy to reveal the nanoscale organization of SARS-CoV-2 replication organelles, showing distinct vgRNA and dsRNA clusters and changes over infection stages.

Exploring masses and internal mass distributions of single carboxysomes in free solution using fluorescence and interferometric scattering in an anti-Brownian trap

Ratiometric Sensing of Redox Environments Inside Individual Carboxysomes Trapped in Solution

The paper improves the ISABEL trap to monitor redox environments inside individual carboxysomes using ratiometric fluorescence.

Fast and parallel nanoscale 3D tracking of heterogeneous mammalian chromatin dynamics

This paper introduces a method for fast and parallel 3D tracking of chromatin dynamics in mammalian cells, revealing heterogeneity and anisotropy in motion patterns and changes upon actin inhibition.

Exploring cell surface-nanopillar interactions with 3D super-resolution microscopy

This paper introduces a 3D single-molecule super-resolution microscopy method to study protein distribution at the nano-bio interface with 10-20 nm precision.

Multi-color super-resolution imaging to study human coronavirus RNA during cellular infection

This study uses multi-color super-resolution imaging to analyze the spatial organization of coronavirus RNA during infection and assess antiviral drug effects.

ATP-responsive biomolecular condensates tune bacterial kinase signaling

The paper shows ATP depletion enhances kinase activity via phase separation in bacterial condensates, enabling robust signaling under nutrient stress.

Continuous, Topologically Guided Protein Crystallization Controls Bacterial Surface Layer Self-Assembly

This study shows that protein self-assembly alone can form and maintain bacterial surface layers, guided by surface topology without enzymatic assistance.

Revealing the nanoscale morphology of the primary cilium using super-resolution fluorescence microscopy

This study uses super-resolution microscopy and computational methods to quantify nanoscale morphology changes in primary cilia under different treatments.

Phospho-signal flow from a pole-localized microdomain spatially patterns transcription factor activity

The paper studies how a microdomain at the cell pole of Caulobacter crescentus spatially patterns transcription factor activity through phospho-signaling.

Spatial organization and dynamics of RNase E and ribosomes in Caulobacter crescentus

This study uses microscopy to show RNase E and ribosomes are spatially organized in Caulobacter crescentus, with clustering linked to RNA availability and rRNA gene location.

Single-molecule diffusometry reveals the nucleotide-dependent oligomerization pathways of Nicotiana tabacum Rubisco activase

This study uses single-molecule diffusometry to quantify the nucleotide-dependent oligomerization pathways of Nt-Rca, revealing concentration-dependent assembly and subunit exchange rates.

3D Single-Molecule Super-Resolution Microscopy With A Tilted Light Sheet

This paper introduces TILT3D, a 3D single-molecule super-resolution microscopy technique that enables high-precision imaging in thick cells using tilted light sheets and specialized PSFs.