Author

L. Shackleford

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

Click to filter author papers

Bioengineering· 1 Synthetic Biology· 1

Signature Assays, Organisms & Methods

Click to filter · or open tag

Organism:Arabidopsis· 1 Organism:C. elegans· 1 Approach:experimental assay· 1 Approach:genetic perturbation / CRISPR· 1

Frequent Co-Authors

D.-K. Purusothaman· 2 shared L. Alphey· 2 shared T. Harvey-Samuel· 2 shared E. Gonzalez· 1 shared J. X. D. Ang· 1 shared K. Esvelt· 1 shared K. Nevard· 1 shared M. A. E. Anderson· 1 shared

Activity by Year

2021-2022 · peak 1/yr

Co-Author Collaboration Network

10 top collaborators · 39 mutual co-author links

Click any collaborator node to inspect or filter shared papers

Focal author (center)Selected co-authorORCID co-authorUnlinked name

Curved chords = mutual co-authorship

2 shared papersSynthetic Biology

D.-K. Purusothaman

Mutual co-authors in cluster

Author profile →

2 papers

Recent·Most cited

A multiplexed, confinable CRISPR/Cas9 gene drive propagates in caged Aedes aegypti populations

M. A. E. Anderson, E. Gonzalez, M. P. Edgington, J. X. D. Ang, D.-K. Purusothaman, L. Shackleford, K. Nevard, S. A. N. Verkuijl, T. Harvey-Samuel, P. T. Leftwich, K. Esvelt, L. Alphey

The paper tests a CRISPR/Cas9 gene drive in caged Aedes aegypti populations, showing it increases from 50% to 89% carrier frequency despite fitness costs.

bioRxiv · Aug 12, 2022 · PreprintSynthetic Biology

CRISPR/Cas-9 mediated knock-in by homology dependent repair in the West Nile Virus vector Culex quinquefasciatus Say

D.-K. Purusothaman, L. Shackleford, M. Anderson, T. Harvey-Samuel, L. Alphey

This paper demonstrates the first successful germline gene knock-in in Culex quinquefasciatus using CRISPR/Cas9 and homology-dependent repair, achieving a 2.8% transformation rate with a recessive lethal phenotype.

bioRxiv · Jan 14, 2021 · PreprintBioengineering